Перегляд за Автор "Laziuka, Yuliya"

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Biosynthesis and characteriсtics of silver nanoparticles obtained using Saccharomyces cerevisiae М437
(2021) Skrotska, Oksana; Kharchenko, Yevgen; Laziuka, Yuliya; Marynin, Andriy; Kharchuk, Maksym
Due to the wide antimicrobial spectrum, silver nanoparticles (AgNPs) have great potential for use in the food industry to control foodborne pathogens. The culture supernatant and cell-free aqueous extract from biomass Saccharomyces cerevisiae M437 were used for the synthesis of AgNPs. The fact of the synthesis of biogenic AgNPs was confirmed by analysing the absorption spectra of the samples in the range of 200-700 nm. The size and zeta potential of AgNPs were determined using Zetasizer Nano ZS. The morphology of nanoparticles was examined using electron microscopy. Using spectral analysis in the UV-visible region, it was confirmed the formation of AgNPs in the investigated solutions. A pronounced absorption peak of AgNPs obtained using a cell-free aqueous extract from S. cerevisiae M437 was recorded in the wavelength range from 300 to 540 nm with a peak at 425 nm. For nanoparticles obtained using the supernatant, a widening spectral range of absorption was observed, which may be associated with the aggregation of AgNPs. AgNPs synthesized using the supernatant S. cerevisiae M437 had a spherical shape with a diameter of about 15 nm. The polydispersity index (PdI) of AgNPs solutions was 0.3, and the zeta potential was 13.6 mV. After storage for 45 days at 4 °C, the PdI value increased 1.6 times, and the zeta potential increased by 11.7 %. This may indicates a possible change in the shape of AgNPs, the formation of an agglomerate, or other processes that takes place in a colloidal solution during storage. AgNPs that were obtained using a cell-free aqueous extract from biomass of S. cerevisiae M437 had an oval shape with a size of 21.3×14.2 nm. The PdI and zeta potential values were similar to the nanoparticles obtained using the supernatant. However, after storage, these values differed significantly: the value of PdI increased 1.3 times, and the zeta potential decreased by 29%. So, the solution of silver nanoparticles obtained in this way is more stable after storage under the specified conditions. The possibility of extracellular synthesis of silver nanoparticles using the yeast Saccharomyces cerevisiae M437 has been shown. The shape, size, and zeta potential of biogenic AgNPs are described and their stability after storage is proved.
Biosynthesis of silver nanoparticles by Saccharomyces cerevisiae M437
(2021) Kharchenko, Yevgen; Skrotska, Oksana; Laziuka, Yuliya; Marchenko (Potapenko), Valeria
Silver nanoparticles (AgNPs) are widely used in medicine and pharmaceuticals as biosensors, diagnostic agents, targeted drug delivery agents with controlled release, as antimicrobial, and anticancer agents. AgNPs are mainly produced by chemical synthesis. The disadvantages of this method: the use of toxic substances (ethylene glycol, hydrazine, and sodium borohydride), low yield of nanoparticles, expensive isolation, and purification. Therefore, it is important to find a safe and effective synthesis method, for example using green synthesis. Biosynthesis can provide a high yield of particles stabilized by natural substances that have high biocompatibility. Biological synthesis can take place both intracellular and extracellular. Cell extracts can also be used for biosynthesis. In this study, the purpose was to synthesize silver nanoparticles using metabolites contained in the supernatant of Saccharomyces cerevisiae M437.
Biosynthesis of silver nanoparticles using Acinetobacter calcoaceticus ІМV V-7241 cell-free supernatant
(2020) Laziuka, Yuliya; Kharchenko, Yevgen; Skrotska, Oksana
Currently, the biological synthesis of nanoparticles and their properties and application in the fields of medicine and the environment is an important area of nanotechnology. Synthesis of nanoparticles using chemical and physical methods has several disadvantages. These drawbacks include the use of toxic solvents, the formation of dangerous by-products and high energy consumption. Therefore, it is important to obtain nanoparticles in a biogenic way, in particular by microorganisms. The mechanisms of biogenic synthesis of nanoparticles have not been fully studied. There are reports on the role of surface active agents (surfactants) in this process. Therefore, the producer of surfactants Acinetobacter calcoaceticus ІМВ В-7241 (which was isolated from the oil contaminated environment) was selected for the study.
Ecological synthesis of silver nanoparticles using bacteria
(2020) Laziuka, Yuliya; Skrotska, Oksana; Kharchenko, Yevgen
Due to the potential use of nanomaterials, there is a need to investigate environmentally friendly technologies for the synthesis of nanoparticles. Traditionally, nanoparticles are synthesized by physical and chemical methods. However, these methods are characterized by the use of hazardous and aggressive substances, which are harmful to the environment and cause serious health problems. It should be noted that the production of nanoparticles using microorganisms, in particular bacteria, is environmentally safe, since this method of synthesis does not require the use of toxic materials.