Перегляд за Автор "Lych (Tkachenko), Inna"

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Adjuvants for the construction anticancer vaccines based on chick embryonic proteins
(2014) Gregirchak, Natalia; Lych (Tkachenko), Inna
It was chosen potential excipients for the design of cancer vaccines and study their effects on the immune system in experimental animals with Lewis lung carcinoma.
Bacteriocins Lactobacillus – an alternative to antimicrobial drugs
(2021) Voloshina, Iryna; Soloshenko, Kateryna; Krasinko, Victoria; Lych (Tkachenko), Inna; Shkotova, Lyudmila
The review presents the characteristics of bacteria of the Lactobacillus family and their ability to synthesize various bacteriocins. The classification of lactobacilli bacteriocins is given, which includes three classes: class I – lantibiotics (peptides with a molecular weight of less than 5 kDa, which containing lanthionine), class II – unmodified bacteriocins, also called non-lantibiotics (heat-resistant peptides, which do not contain lanthionine and have a molecular weight of less 10 kDa) and class III – a poorly studied group of thermolabile proteins with a molecular weight of more than 30 kDa. Lactobacilli are shown to synthesize a wide spectrum of bacteriocins, which demonstrate a variety of actions and are able to inhibit the growth of numerous species of opportunistic gram-positive microflora. The article also provides the examples of lactobacilli bacteriocins isolated from food products (fermented meat, fish, kombucha, goat milk, koumiss, etc.) and various human biotopes (microbiota of breast milk, intestinal tract, and vaginal secretions). Additionally, the review shows the prospects of wide application of bacteriocins synthesized by lactobacilli in the food and pharmaceutical industries.
Determination of molecular weights of biologically active fragments of colostrum by electrophoresis method
(2017) Lych (Tkachenko), Inna; Veriovka, Serhii; Voloshina, Iryna
It has been determined that high molecular weight fractions of colostrum contain protein molecules with molecular weights of 160-190 kDa, among which are immunoglobulins. Separate parts of immunoglobulins and other proteins, namely, IgG (55kDa) and IgA (62kDa) heavy chains, secretory component of sIgA, and lactoferrin, have also been found out. The test samples of high molecular weight peptides of colostrum contain the biologically active fragments among which there are mostly immunoglobulins, their parts, proteinsabzymes, other protein fragments. The proteins with the molecular weight of 80, 62, 55 kDa are also determined, they can represent heavy chains (55 kDa) and light chains (25 kDa) of IgG and sIgA. In particular, sIgA is a supermolecular complex consisting of Н- and L-chains of IgA (62, 25 kDa, respectively) and a secretory component (80 kDa). Apart from the secretory component of IgA, the protein molecules with the molecular weight of 80 kDa can be halves of the molecules of immunoglobulins or lactoferrin.
Environmental belief of students in higher technical educational institutions – the path to harmonious relationship between technogenic society and nature
(2011) Skrotska, Oksana; Voloshina, Iryna; Krasinko, Victoria; Lych (Tkachenko), Inna; Teterina, Svitlana
The article is devoted to the consideration of ways of the topical problems decision which get in way of harmonization of relations between technogenic society and the nature. It also devoted to the analysis of state of training of specialists on ecological orientation in the higher technical educational institutions.
Green biosynthesis of zinc nanoparticles
(2022) Rohova, Maryna; Kovalenko, Vladyslav; Tkachenko, Volodymyr; Lych (Tkachenko), Inna; Voloshina, Iryna
Currently there is a growing need for the development of an environmentally friendly process of synthesis of nanoparticles, during which no toxic chemicals are used. That is why an important area of research in nanotechnology sphere is the synthesis of metal nanoparticles by microorganisms such as bacteria and yeast (detoxification often occurs by reduction of metal ions/formation of metal sulfides). Bacteria are the organism of choice due to their fast growth, high efficiency and low cost. Metal nanoparticles exhibit antimicrobial properties, but the properties of nanoparticles depend on their size and shape, making them specific for different applications. Nevertheless, the desired size and shape of nanoparticles can be obtained by optimizing the synthesis process through manipulating their reaction conditions. Microbial synthesis of nanoparticles is an alternative to chemical and physical methods, as it is non-toxic and biocompatible. Despite the relevance of the application of the “green synthesis” method in the field of nanotechnology, biosynthesis by bacterial organisms has certain disadvantages, such as a high probability of pathogenicity, labour-intensive cultivation, and pollution problems. Ultimately, there is a need to explore more potential microorganisms for the synthesis of metal nanoparticles. The paper provides a review of literature data on the biosynthesis of zinc nanoparticles using lactic acid microorganisms Lactobacillus johnsonii, L. gasseri L. paracasei L. acidophilus L. plantarum L. sporogens. It was shown that bacteria are capable of synthesizing both extracellular and intracellular nanoparticles in the wavelength range of 315-392 nm. Data on the manifestation of antimicrobial properties by zinc nanoparticles against various gram-positive and gram-negative bacterial microorganisms and micromycetes: Clostridium difficile, C. perfringens, Escherichia. coli, Salmonella typhi, Candida albicans, Aspergillus flavus, Pseudomonas aeruginosa, Acinetobacter baumanii, Klebsiella pneumonia, Staphylococcus aureus.
Immunotherapeutic approaches in the treatment of herpetic infections
(2012) Skrotska, Oksana; Lych (Tkachenko), Inna; Solominchuk, V.; Zholobak, Nadezhda
The possible consequences of human infecting by Herpesviridae are shown. The basic classes of preparations used in Ukraine for the immunotherapy of Herpesviridae infections, in particular interferons, inductors of interferons and immunomodulators are described. The comparative description these preparations and their advantages and disadvantages are presented.
Methods of assessment of operating effectiveness of the Intellectual information and analytical system of Occupational safety management at pharmaceutical іndustry enterprises
(2019) Siryk, Alina; Evtushenko, Olga; Lych (Tkachenko), Inna; Soloshenko, Kateryna
The article overlooks methodology for evaluating the effectiveness of the intellectual information-analytical system of labor protection management at the enterprises of the pharmaceutical industry. The introduction of this technique at the enterprises of the pharmaceutical industry in the traditional procedures of internal audit will achieve a new quality of functioning of automated information-analytical systems of labor protection management and significantly increase the efficiency of information processing in decision-making processes due to the efficiency and accuracy of choosing the optimal set of measures for ensuring safety.
Polyfunctional properties of goat colostrum proteins and their use
(2020) Soloshenko, Kateryna; Lych (Tkachenko), Inna; Voloshina, Iryna; Shkotova, Lyudmila
The review presents the composition of goat colostrum, antimicrobial, immunomodulatory and antioxidant properties of biologically active proteins of goat colostrum, as well as the prospects of their use in medicine. Due to the presence of a complex of biologically active proteins such as lactoferrin, lysozyme, lactoperoxidase, immunoglobulins, etc., goat colostrum increases the body’s resistance to infectious diseases, strengthens the immune system, has an antioxidant effect and antibacterial activity against a wide range of microorganisms, thereby preventing or delaying chronic diseases associated with the oxidative stress. Additionally, goat colostrum bioactive proteins show antitumor activity, antiatherogenic properties, the ability to lower the blood pressure and efficiency in the treatment of rheumatoid arthritis.
Practical use of goat milk and colostrum
(2021) Voloshina, Iryna; Soloshenko, Kateryna; Lych (Tkachenko), Inna; Shkotova, Lyudmila
This review presents the protein and amino acid composition of both goat colostrums and milk and describes the properties of goat colostrums and milk components. In addition, the prospects of use of goat milk and colostrum in the food and cosmetics industry and the feasibility of use of goat milk for baby feeding are shown. Functional foods produced from goat milk have antioxidant, anti-inflammatory, cardioprotective, antihypertensive and antiatherogenic activities in the human body. Goat milk cosmetics are very useful for maintaining a healthy skin and are effective in treatment of various skin diseases. Infant formula based on goat milk provides comfortable digestion for babies and are better at absorbing proteins, fats and other nutrients than infant formula based on cow’s milk.
Screening adjuvants organic and inorganic nature for constructing cancer vaccines
(2012) Babiy, O.; Gregirchak, Natalia; Lych (Tkachenko), Inna; Shpak, E.
On іntaktnih tvarinah vivchali іmunomodulyuyuchu aktivnіst the heterologous vaccine vigotovlenih on osnovі Kuryachii embrіonalnih bіlkіv that a'dyuvantіv organіchnoї that neorganіchnoї nature. Vstanovleno scho naybіlshim іmunologіchno Activity ad'yuvantami stvorennya for the heterologous vaccine protipuhlinnih Yea glіkoproteїdi B. subtilis B-7025 s MM 18.5 kDa that 70 kDa. Spectrum іmunologіchnih efektіv doslіdzhuvanih vaccines include aktivuyuchy vpliv on klіtinnі i gumoralnі reaktsії adaptive іmunіtetu (aktivnіst CTL spetsifіchna tsitotoksichnіst sirovatki krovі) that on reaktsії prirodnoї protipuhlinnoї rezistentnostі (cytotoxic aktivnіst Lf i Matt). Results of the work yavlyayutsya basis for doslіdzhen away vplivu vaccines humoral Lanka іmunіtetu that mozhlivostі stvorennya protipuhlinnih preparatіv on osnovі danih glіkoproteїdіv that Kuryachii embrіonalnih bіlkіv.
The role of colostrum in congenital immunity
(2016) Borodina, Olga; Tymchuk, Alla; Lych (Tkachenko), Inna
Molosa is a complex biological fluid with a high nutritional and energy value, with a content of biologically active and protective factors that provides a reliable adaptation of the newborn baby to the environment and protection against a range of infections. Molosa is secreted by mammary glands at the end of pregnancy and in the first 2-3 days after childbirth.