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Документ Microbial surface-active substances as antiadhesive agents(2016) Pirog, Tatiana; Savenko, Inga; Lutsay, DariyaThe literature data of recent years about capacity of biosurfactants synthesized by bacteria (Pseudomonas, Lactobacillus, Bacillus) and fungi (Candida, Trichosporon, Saccharomyces) not only to avert the adhesion of microorganisms on the different materials, but also to destroy formed biofilms on them were presented. The perspective of biosurfactants to prevent pathogens colonization on biotic and abiotic surfaces, that is known, can be a reason of cause and spread of infectious diseases was discussed. The data of our researches about antiadhesive properties of biosurfactants synthesized by Acinetobacter calcoaceticus IMV B-7241, Nocardia vaccinii IMV B-7405 and Rhodococcus erythropolis IMV Ac-5017 were presented.Документ Microbial surfactants in environmental technologies(2015) Pirog, Tatiana; Konon, Anastasia; Savenko, IngaIn the review the literature and own experimental data on the use of microbial surface active glycolipids (rhamno-, sophoro- and trehalose lipids) and lipopeptides for water and soil purification from oil and other hydrocarbons, removing toxic heavy metals (Cu2+, Cd2+, Ni2+, Pb2+), degradation of complex pollution (oil and other hydrocarbons with heavy metals), and the role of microbial surfactants in phytoremediation processes was shown. The factors that limit the use of microbial surfactants in environmental technologies are discussed. Thus, at certain concentrations biosurfactant can exhibit antimicrobial properties and inhibit microorganisms destructing xenobiotics. Microbial biodegradability of surfactants may also reduce the effectiveness of bioremediation.Документ Technologies of synthesis of organic substances by microorganisms using waste biodiesel production(2015) Pirog, Tatiana; Gritsenko (Manzhula), Natalia; Sofіlkanich (Morozova), Anna; Savenko, IngaWe describe here literature and our experimental data concerning microbial synthesis using waste biodiesel production, mono- and dihydric alcohols (1,3-propanediol, 2,3-butanediol, butanol, ethanol), polyols (mannitol, erythritol, arabitol), organic acids (citric, succinic, lactic, glyceric), polymers and compounds with a complex structure (polysaccharides, polyhydroxyalkanoates, surfactants, cephalosporin, cyanocobalamin). In some mentioned cases recombinant producer strains were used. It was shown that due to the presence of potential inhibitors in the composition of technical (crude) glycerol (methanol, sodium and potassium salts), the efficiency of synthesis of most microbial products on such a substrate is lower than on the purified glycerol. However, the need of utilization of this toxic waste (storage and processing of crude glycerol is a serious environmental problem due to the high alkalinity and the content of methanol in it), compensates the lower rates of synthesis of the final product. Furthermore, currently considering the volumes of crude glycerol formed during the production of biodiesel, microbial technologies are preferred for its utilization, allowing realizing biosynthesis of practically valuable metabolites in the environment with the highest possible concentration of this waste. Using of crude glycerol as a substrate will reduce the cost of products of microbial synthesis and increase the profitability of biodiesel production.Документ The effect of Аcinetobacter calcoaceticus IMV B-7241 surfactantas on microbial adhesion to abiotic surfaces(2014) Savenko, Inga; Pirog, Tatiana; Skrotska, OksanaWe have studied the effect of surface-active substances (SAS, surfactants) of Acinetobacter calcoaceticus IMV B-7241 with various degree of purification (the supernatant of culture liquid, the solution of SAS, 0,001-0,036 mg/ml) for the attachment of bacteria and fungi to abiotic surfaces. The degree of adhesion of the test-cultures depended on the material’s type and the concentration of SAS in the preparations. The preparation 1 (supernatant) with the concentration of SAS 0,005-0,009 mg/ml was the more effective: after treatment of the abiotic materials with this preparation the number of attached cells of bacteria and fungi decreased on average 45-60 %.Документ The role of Acinetobacter Calcoaceticus IMV В-7241 surfactants in biofilms destruction(2016) Savenko, Inga; Ivahniuk, Mykola; Pirog, Tatiana; Smirnova, Elizabeth