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Документ Antimicrobial activity of surfactances of bacteria Nocardia, Rhodococcus and Acinetobacter genera(2021) Pirog, Tatiana; Kliuchka, Igor; Lutsay, Dariya; Kliuchka (Nykytyuk), Lilia; Skrotska, OksanaIt was found that the minimum inhibitory concentrations against bacteria and yeast of Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017 and Nocardia vaccinii IMV B-7405 surfactants, synthesized on traditional substrates, were 9–120 μg/ml and were within the limits defined for the surfactants known in the world. It was for the first time established that surfactants synthesized by the study strains on wastes of biodiesel production and fried sunflower oil were characterized by high antimicrobial activity against bacteria and yeast (minimum inhibitory concentrations 0.45–120 and 1.9–142 μg/ml respectively). It was found that the added of both live and inactivated Escherichia coli ІEM-1 and Bacillus subtilis BT-2 cells in R. erythropolis IMV Ac-5017 and N. vaccinii IMV B-7405 medium cultivation was accompanied by synthesis of surfactants, minimum inhibitory concentrations of which were several times lower than those showed for surfactants synthesized without competitive microorganisms. The obtained results indicate the possibility of using the studied surfactants as effective antimicrobial agents.Документ Biosynthesis and characteriсtics of silver nanoparticles obtained using Saccharomyces cerevisiae М437(2021) Skrotska, Oksana; Kharchenko, Yevgen; Laziuka, Yuliya; Marynin, Andriy; Kharchuk, MaksymDue to the wide antimicrobial spectrum, silver nanoparticles (AgNPs) have great potential for use in the food industry to control foodborne pathogens. The culture supernatant and cell-free aqueous extract from biomass Saccharomyces cerevisiae M437 were used for the synthesis of AgNPs. The fact of the synthesis of biogenic AgNPs was confirmed by analysing the absorption spectra of the samples in the range of 200-700 nm. The size and zeta potential of AgNPs were determined using Zetasizer Nano ZS. The morphology of nanoparticles was examined using electron microscopy. Using spectral analysis in the UV-visible region, it was confirmed the formation of AgNPs in the investigated solutions. A pronounced absorption peak of AgNPs obtained using a cell-free aqueous extract from S. cerevisiae M437 was recorded in the wavelength range from 300 to 540 nm with a peak at 425 nm. For nanoparticles obtained using the supernatant, a widening spectral range of absorption was observed, which may be associated with the aggregation of AgNPs. AgNPs synthesized using the supernatant S. cerevisiae M437 had a spherical shape with a diameter of about 15 nm. The polydispersity index (PdI) of AgNPs solutions was 0.3, and the zeta potential was 13.6 mV. After storage for 45 days at 4 °C, the PdI value increased 1.6 times, and the zeta potential increased by 11.7 %. This may indicates a possible change in the shape of AgNPs, the formation of an agglomerate, or other processes that takes place in a colloidal solution during storage. AgNPs that were obtained using a cell-free aqueous extract from biomass of S. cerevisiae M437 had an oval shape with a size of 21.3×14.2 nm. The PdI and zeta potential values were similar to the nanoparticles obtained using the supernatant. However, after storage, these values differed significantly: the value of PdI increased 1.3 times, and the zeta potential decreased by 29%. So, the solution of silver nanoparticles obtained in this way is more stable after storage under the specified conditions. The possibility of extracellular synthesis of silver nanoparticles using the yeast Saccharomyces cerevisiae M437 has been shown. The shape, size, and zeta potential of biogenic AgNPs are described and their stability after storage is proved.Документ Biosynthesis of recombinant interferons in transgenic plants(2017) Holubiev, Pavlo; Kharchenko, Yevgen; Skrotska, OksanaThe production of recombinant cytokines with transgenic plants may solve the problem of high demand on drugs against severe diseases, such as hepatitis, AIDS, chronic granulomatous disease, etc. Microorganisms, mammal cell cultures and even plants may be involved in interferon (IFN) production. Considering an array of disadvantages of microbial and mammalian cell cultures, transgenic plants become an object of interest as a perspective producer of interferon.Документ Biosynthesis of silver nanoparticles by Saccharomyces cerevisiae M437(2021) Kharchenko, Yevgen; Skrotska, Oksana; Laziuka, Yuliya; Marchenko (Potapenko), ValeriaSilver nanoparticles (AgNPs) are widely used in medicine and pharmaceuticals as biosensors, diagnostic agents, targeted drug delivery agents with controlled release, as antimicrobial, and anticancer agents. AgNPs are mainly produced by chemical synthesis. The disadvantages of this method: the use of toxic substances (ethylene glycol, hydrazine, and sodium borohydride), low yield of nanoparticles, expensive isolation, and purification. Therefore, it is important to find a safe and effective synthesis method, for example using green synthesis. Biosynthesis can provide a high yield of particles stabilized by natural substances that have high biocompatibility. Biological synthesis can take place both intracellular and extracellular. Cell extracts can also be used for biosynthesis. In this study, the purpose was to synthesize silver nanoparticles using metabolites contained in the supernatant of Saccharomyces cerevisiae M437.Документ Biosynthesis of silver nanoparticles using Acinetobacter calcoaceticus ІМV V-7241 cell-free supernatant(2020) Laziuka, Yuliya; Kharchenko, Yevgen; Skrotska, OksanaCurrently, the biological synthesis of nanoparticles and their properties and application in the fields of medicine and the environment is an important area of nanotechnology. Synthesis of nanoparticles using chemical and physical methods has several disadvantages. These drawbacks include the use of toxic solvents, the formation of dangerous by-products and high energy consumption. Therefore, it is important to obtain nanoparticles in a biogenic way, in particular by microorganisms. The mechanisms of biogenic synthesis of nanoparticles have not been fully studied. There are reports on the role of surface active agents (surfactants) in this process. Therefore, the producer of surfactants Acinetobacter calcoaceticus ІМВ В-7241 (which was isolated from the oil contaminated environment) was selected for the study.Документ Ecological producing silver nanoparticles(2022) Marchenko (Potapenko), Valeria; Skrotska, OksanaIn recent times there are several ways to obtain silver nanoparticles. Among which there is a biological method using microorganisms or plants which is more environmentally friendly compared to other methods. When using a cell-free aqueous yeast extract with parameters of 24 hours of cultivation of Saccharomyces cerevisiae M437 and 24 hours biosynthesis silver nanoparticles were obtained.Документ Ecological synthesis of silver nanoparticles using bacteria(2020) Laziuka, Yuliya; Skrotska, Oksana; Kharchenko, YevgenDue to the potential use of nanomaterials, there is a need to investigate environmentally friendly technologies for the synthesis of nanoparticles. Traditionally, nanoparticles are synthesized by physical and chemical methods. However, these methods are characterized by the use of hazardous and aggressive substances, which are harmful to the environment and cause serious health problems. It should be noted that the production of nanoparticles using microorganisms, in particular bacteria, is environmentally safe, since this method of synthesis does not require the use of toxic materials.Документ Environmental belief of students in higher technical educational institutions – the path to harmonious relationship between technogenic society and nature(2011) Skrotska, Oksana; Voloshina, Iryna; Krasinko, Victoria; Lych (Tkachenko), Inna; Teterina, SvitlanaThe article is devoted to the consideration of ways of the topical problems decision which get in way of harmonization of relations between technogenic society and the nature. It also devoted to the analysis of state of training of specialists on ecological orientation in the higher technical educational institutions.Документ Immunotherapeutic approaches in the treatment of herpetic infections(2012) Skrotska, Oksana; Lych (Tkachenko), Inna; Solominchuk, V.; Zholobak, NadezhdaThe possible consequences of human infecting by Herpesviridae are shown. The basic classes of preparations used in Ukraine for the immunotherapy of Herpesviridae infections, in particular interferons, inductors of interferons and immunomodulators are described. The comparative description these preparations and their advantages and disadvantages are presented.Документ Practically valuable metabolites of marine microorganisms(2020) Pirog, Tatiana; Martyniuk, Anna; Skrotska, Oksana; Shevchuk, TetianaThe review considers the modern literature data on the synthesis by fungi, actinobacteria, and bacteria isolated from marine ecosystems (seawater, bottom sediments, flora and fauna, mangrove biomes, glaciers), practically valuable metabolites. Marine microorganisms synthesize a wide range of practically valuable enzymes (cold-active galactosidase, agarase, alginate lyase, fucoidase, chitinase, etc.), surface-active glyco- and lipopeptides with emulsifying, antimicrobial and antiadhesive activity, as well as secondary metabolites with diverse biological activity (antimicrobial, antitumor, cytotoxic). However, the use of marine producers in biotechnological processes is constrained by their low synthesizing capacity and high costs of biosynthesis (complex nutrient media and expensive carbohydrate substrates). In biotechnology, marine microorganisms can be used as sources of genes encoding the synthesis of new biologically active substances with unique properties, including antimicrobial and antitumor.Документ Practically valuable properties of the surfactant synthesized by Rhodococcus genus actinobacteria(2020) Pirog, Tatiana; Petrenko, Natalia; Skrotska, Oksana; Paliichuk, Olesia; Shevchuk, Tetiana; Iutynska, GalynaCurrently, microbial surfactants are the objects of intense research because of their surface-active and emulsifying properties, high antimicrobial, anti-adhesive activity, and ability to destroy biofilms. The review provides current literature data on the properties of surfactants synthesized by Rhodococcus genus actinobacteria, determining their practical significance. The researchers’ interest in the surfactants of Rhodococcus bacteria is primarily due to their key role in the destruction of xenobiotics (aliphatic, heterocyclic and polycyclic aromatic hydrocarbons). Information on the antimicrobial and anti-adhesive activity of surfactants of Rhodococcus genus bacteria remains scarce at present, while the immunomodulatory properties of these products of microbial synthesis are studied more actively than for other microbial surfactants known in the world. The data of our experimental studies on the practically valuable properties of surfactants synthesized by Rhodococcus erythropolis IMV Ac-5017 are presented. Unlike surfactants of other representatives of Rhodococcus genus bacteria, surfactants of IMV As-5017 strain are multifunctional preparations. Because in addition to the high efficiency of the destruction of oil pollution, including complex with heavy metals, surfactants are characterized by high antimicrobial and anti-adhesive activity, including the ability to destroy biofilms.Документ Saccharomycetes as biological agents for the mediated biosynthesis of silver nanoparticles(2021) Kharchenko, Yevgen; Skrotska, OksanaThe most commonly used are chemical and physical methods of silver nanoparticles (AgNPs) synthesis, but there is also biological synthesis, which is a promising and more environmentally friendly method. Synthesized using the supernatant Saccharomycescerevisiae M437 AgNPs had a spherical shape. AgNPs that were obtained using a cell-free aqueous extract of S. cerevisiae M437 had an oval shape.Документ Technological aspects of probiotics obtaining(2014) Starovoitova, Svitlana; Skrotska, OksanaСurrent groups of drugs using for correction of the humans normal microflora were reviewed. The types of problems and promising directions for improving probiotics were shown. Different probiotic's drug-forms were considered, and the possible aspects of the efficiency of bacterial agents were shown. Technological methods to improve technologies of obtaining and extending the shelf life of probiotic were analyzed. The technology of polystrain substance of probiotic with high therapeutic properties and spread spectrum of therapeutic action were shownДокумент The effect of Аcinetobacter calcoaceticus IMV B-7241 surfactantas on microbial adhesion to abiotic surfaces(2014) Savenko, Inga; Pirog, Tatiana; Skrotska, OksanaWe have studied the effect of surface-active substances (SAS, surfactants) of Acinetobacter calcoaceticus IMV B-7241 with various degree of purification (the supernatant of culture liquid, the solution of SAS, 0,001-0,036 mg/ml) for the attachment of bacteria and fungi to abiotic surfaces. The degree of adhesion of the test-cultures depended on the material’s type and the concentration of SAS in the preparations. The preparation 1 (supernatant) with the concentration of SAS 0,005-0,009 mg/ml was the more effective: after treatment of the abiotic materials with this preparation the number of attached cells of bacteria and fungi decreased on average 45-60 %.