Перегляд за Автор "Sofіlkanich (Morozova), Anna"

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Antimicrobial activity of exocellular metabolites of Acinetobacter Calcoaceticus IMB B-7241, Rhodococcus Erythropolis IMB Ac-5017, Nocardia
(2012) Konon, Anastasia; Sofіlkanich (Morozova), Anna; Pirog, Tatiana; Beregova (Pokora), Khrystyna; Chebotaryova, Ksenia
It was showed that exocellular metabolites of strains IMV B-7241, IMV Ac-5017 and K-8 inherent antimicrobial properties against some phytopathogenic microorganisms. Therefore, these preparations can be used as environmentally safe antimicrobial products, which exhibit high efficiency against a number of pathogenic bacteria resistant to existing traditional preparations.
Biosurfactants of Rhodococcus erythropolis IMV Ас-5017: synthesis intensification and practical application
(2013) Pirog, Tatiana; Sofіlkanich (Morozova), Anna; Shulyakova, Mariya; Shevchuk, Tetiana
Intensification of the surfactant synthesis by Rhodococcus erythropolis IMV Ac-5017 on different substrates, including industrial waste, as well as the use of surfactant preparations for oil degradation were studied. It was established that the addition of fumarate (0.2 %) and citrate (0.1 %) into the medium with ethanol, n-hexadecane, or glycerol (1–2 %) was accompanied by an increase of conditional surfactant concentration by 1.5–1.7 times compared to the indexes in the medium without organic acids. The intensification of surfactant synthesis in the presence of fumarate and citrate is caused by the increased activity of isocitrate lyase (by 1.2–15-fold) and enzymes of the surfactant biosynthesis (by 2–4.8-fold) compared to their activity in the medium without precursors. The possibility of surfactant synthesis intensification (by 3–4-fold) while cultivating of R. erythropolis IMV Ac-5017 in the medium with oil containing substrates (2 %) and glucose (0.1 %) was shown. The introduction of 0.01 mM Cu2+ in the exponential growth phase of strain IMV Ac-5017 in the medium with ethanol accompanied by the increasing conditional surfactant concentration by 1.9 times. The highly efficient remediation (92–95 %) of oil (2–2.6 g/L) and Cu2+ polluted water after treatment with surfactant preparations (native cultural liquid) at low concentrations (5 %) was determined.
Intensification of surfactants’ synthesis by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii K-8 on fried oil and glycerol containing medium
(2013) Pirog, Tatiana; Sofіlkanich (Morozova), Anna; Konon, Anastasia; Shevchuk, Tetiana; Ivanov, Sergey
Актуальною проблемою сьогодення є пошук шляхів для переробки відходів. Біотехнологія є одним з найбільш екологічно привабливих методів, що дають змогу вирішити проблему утилізації відходів і одержання цінних мікробних продуктів, наприклад, поверхнево-активних речовин (ПАР). Встановлено, пересмажена (відпрацьована) соняшникова олія, олієвмісні відходи (фузи) і гліцерин можуть бути використані для синтезу ПАР Rhodococcus erythropolis IMВ Ac-5017, Acinetobacter calcoaceticus ІМВ В-7241 і Nocardia vaccinii К-8. Внесення глюкози (0,1%) у середовище з відпрацьованою олією (2% об.) супроводжувалося збільшенням у 4 рази кінцевої концентрації поверхнево-активних речовин (6,8 г/л). У разі одночасного добавлення фумарату і цитрату (0,01-0,2%) у середовище культивування штамів IMВ B-7241 і К-8 спостерігали підвищення кількості позаклітинних ПАР у 2-2,5 рази порівняно з вирощуванням без органічних кислот. Збільшення концентрації ПАР штаму IMВ B-7241 зумовлене одночасним функціонуванням двох анаплеротичних шляхів, а також підвищенням у 3-5 разів активності ферментів біосинтезу поверхнево-активних речовин. Культивування на суміші гліцерину і н-гексадекану (0,5-1,0 об.%) супроводжувалося підвищенням у 1,5-3 рази синтезу ПАР. Препарати ПАР штамів IMВ B-7241 (0,15-0,22 мг/мл) і IMВ Ac-5017 (0.61-2.1 мг/мл) проявляли антимікробну активність щодо Escherichia coli IEM-1 (загибель 67% клітин), а також вегетативних клітин (загибель 45-100%) і спор (загибель 75%) Bacillus subtilis БТ-2. Searching for the ways to process waste has become very topical today. Biotechnology is one of the most environmentally attractive methods, which has the ability to solve the problem of waste utilization and to produce the valuable microbial products, for example biosurfactants. We concluded that fried sunflower oil, oil-containing wastes (soapstock) and glycerol can be used for biosurfactant production by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii K-8. Glucose addition (0.1%) into the medium with fried oil (2 vol.%) led to a 4-fold increase of final surfactant concentration (6.8 g/L). The simultaneous addition of fumarate and citrate (0.01–0.2%) into the IMV B-7241 and K-8 strains’ cultivating medium was accompanied by an increase of the exocellular biosurfactant quantity by 2–2.5-fold compared to the cultivation without organic acids. An increase in surfactant concentration of IMV B-7241 strain was the result of the simultaneous functioning of two anaplerotic pathways, also resulting in a 3–5-fold increase in activity of biosynthesis enzymes. Cultivating on a mixture of glycerol and n-hexadecane (0.5–1.0 vol.%) led to a 1.5–3-fold increasing surfactant synthesis. Biosurfactant preparations of IMV B-7241 (0.15–0.22 mg/mL) and IMV Ac-5017 (0.61–2.1 mg/mL) strains were effective against Escherichia coli IEM-1 (67% of cell loss), and vegetative (45–100% of cell loss) and spore (75% of spore loss) cells of Bacillus subtilis BT-2.
Intensification of surfactants’ synthesis on fried oil and glycerol containing medium
(2012) Pirog, Tatiana; Sofіlkanich (Morozova), Anna; Konon, Anastasia; Beregova (Pokora), Khrystyna
The possibility of waste use for biosurfactant production was determined. Presence of fumarate and citrate intensified biosurfactant synthesis by 2.5-fold. It was shown that C4-dicarboxylic acids enhanced activity of surfactant biosynthesis enzymes. Cultivation on mixture of substrates resulted in increased surfactants quantity. Досліджено можливість використання відходів для виробництва мікробних поверхнево-активних (ПАР). Наявність фумарату і цитрату призводило до збільшення синтезу ПАР у 2,5 рази. Показано, що С4-дикарбонові кислоти підвищують активність ферментів біосинтезу ПАР. Крім того, збільшення кількості поверхнево-активних речовин спостерігали при культивуванні на суміш субстратів.
Nocardia vaccinii К-8 and acenitobacter calcoaceticus ІМV В-7241 – potential destructors of aromatic compounds
(2012) Antoniuk, Svitlana; Sofіlkanich (Morozova), Anna; Pirog, Tatiana
It is shown that Nocardia vaccinii K-8 and Acenitobacter calcoaceticus IMV B-7241 are able to assimilate aromatic compounds, as well as tosynthesize practically valuable surfactants. That’s why these strains are promisingfor usein the remediation of water and soil polluted with crude oilandaromatic xenobiotics. Показано, що Nocardia vaccinii К-8 та Acenitobacter calcoaceticus ІМВ В-7241 здатні не тільки асимілювати ароматичні сполуки, а й синтезувати при цьому практично цінні поверхнево-активні речовини, завдяки чому є перспективними для використання в очищенні довкілля від комплексних забруднень як нафтопродуктами, так і ксенобіотиками ароматичної природи.
Technologies of synthesis of organic substances by microorganisms using waste biodiesel production
(2015) Pirog, Tatiana; Gritsenko (Manzhula), Natalia; Sofіlkanich (Morozova), Anna; Savenko, Inga
We describe here literature and our experimental data concerning microbial synthesis using waste biodiesel production, mono- and dihydric alcohols (1,3-propanediol, 2,3-butanediol, butanol, ethanol), polyols (mannitol, erythritol, arabitol), organic acids (citric, succinic, lactic, glyceric), polymers and compounds with a complex structure (polysaccharides, polyhydroxyalkanoates, surfactants, cephalosporin, cyanocobalamin). In some mentioned cases recombinant producer strains were used. It was shown that due to the presence of potential inhibitors in the composition of technical (crude) glycerol (methanol, sodium and potassium salts), the efficiency of synthesis of most microbial products on such a substrate is lower than on the purified glycerol. However, the need of utilization of this toxic waste (storage and processing of crude glycerol is a serious environmental problem due to the high alkalinity and the content of methanol in it), compensates the lower rates of synthesis of the final product. Furthermore, currently considering the volumes of crude glycerol formed during the production of biodiesel, microbial technologies are preferred for its utilization, allowing realizing biosynthesis of practically valuable metabolites in the environment with the highest possible concentration of this waste. Using of crude glycerol as a substrate will reduce the cost of products of microbial synthesis and increase the profitability of biodiesel production.
Use of cells and metabolitesof Nocardia vaccinii k-8 in bioremediation processes
(2012) Sofіlkanich (Morozova), Anna; Gritsenko (Manzhula), Natalia; Pirog, Tatiana
Рossibility of effective use of cells and surfactants preparations of Nocardia vaccinii K-8 in environmental technologies for bioremediation of oil polluted ecosystemswas shown.
Use of cells and surfactants of Nocardia vaccinii K-8 in bioremediation processes
(2012) Gritsenko (Manzhula), Natalia; Sofіlkanich (Morozova), Anna; Konon, Anastasia
The possibility of use of Nocardia vaccinii K-8 cells as well as their metabolites for remediation of oil polluted ecosystems was studdied. It was shown that the highest oil destruction degree (94-98 %) in polluted water (2.6 g/L) was achieved in the case of treatment with suspension of N. vaccinii K-8 cells (9.8107 CFU/mL) after 30 days, while surfactant preparation of post fermentative cultural liquid (100300 mL/kg) was more effective for remediation (destruction of 74-83 % of oil) of oil polluted soil (20 g/kg). Furthermore, the introduction of 30 ml of these preparation to the oil polluted sand (0.1 mL of oil/1 g of sand) resulted in detachment of 90 % of oil.
Water remediation by biosurfactant preparation of Rhodococcus erythropolis IMV Ac-5017 in presence of Cu2+
(2012) Sofіlkanich (Morozova), Anna; Filyuk, Iryna; Pirog, Tatiana
It was showed that the oil destruction degree have increased significantly in presence of Cu2+ and surfactant preparation of Rhodococcus erythropolisІМV Ac-5017 strain. The oil destruction intensification in presence of Cu2+ could be caused by Cu2+positive influence on alcanehydroxylase activity in ІМV Ac-5017 strain, as well as in indigenous microflora.