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Документ Trends and expected benefits of the breaking edge food technologies in 2021–2030(2021) Ivanov, Volodymyr M.; Shevchenko, Oleksandr; Marynin, Andriy; Stabnikov, Victor; Stabnikova, Elena; Gubenia, Oleksii; Shevchenko, Anastasiia; Gavva, Oleksandr M.; Salyuk, AnatolyThe review considered the major trends in the world development of new food processing technologies in 2021–2030: more strict regulations of food safety including QPCR and DNA-sequencing detection of emerging food-borne pathogens, comprehensive control of minor chemical pollutants of food; production of functional food including food for babies, children, pregnant women, elderly, for sport activities, military food, meals ready-to-eat, microelements-enriched food, nutraceuticals, and clinical food; emerging environmentally-friendly and energy-saving food processing; biotechnological food processing using enzymes proteinases, glutamine transferases, galactosidases, enzymes, microbial metabolites, and new sources of food such as insects and artificial meat; personalization of food processing and distribution including adaptation of the food processing to the nutritional needs of different customer groups.Документ The structure of bacterial cell cycle and age structure of bacterial populations(1995) Ivanov, Volodymyr M.; Svechnikova, Tatyana; Stabnikova, Elena; Gregirchak, NataliaВивчення синхронних і асинхронних культур Bacillus'tnegateriutn, Bacillus thuringiensis та Bacillus Ucheniformis показало, що тривалість періоду реплікації хромосомної ДНК (С-період) пропорційна часу генерації,а час між двома циклами реплікації ДНК (що зветься І-періодом) майже постійний та становить 0,5—1,0 год при варіації часу генерації від 1,5 до 2,75 год. Клітинний цикл (період між клітинними поділами) та цикл реплікації ДНК не синхронізовані між собою. Тому тривалість В-періоду (час між термінацією клітинного поділу та ініціацією реплікації ДНК),а також D-періоду (час між термінацією реплікації ДНК та ініціацією клітинного поділу) виявляються як стохастичні параметри в експериментах по синхронному культивуванню. Запропоновані теоретичні моделі клітинного циклу бактерій та вікові структури бактеріальних популяцій. Дані, що випливають з теоретичних моделей, узгоджуються з експериментальними даними проточного цитофлуорометричного аналізу синхронних та асинхронних культур бацил.Документ Improvement of sludge quality by iron-reducing bacteria(2004) Ivanov, Volodymyr M.; Wang, J.-Y.; Stabnikov, Victor; Xing, Zikun; Tay, JooSewage sludge can be used in agriculture as organic fertilizer. However, one of the obstacles for this use is the high concentration of heavy metals and the presence of sulphides (acidifying soil or compost). The aim of this research was to develop the biotechnology for improving the quality of sewage sludge that would be used as organic fertilizer. Microbial reduction of inexpensive sources of Fe(III) in anaerobic digester is proposed as a means of preventing the accumulation of sulphide and of enhancing the accumulation of phosphate in sewage sludge. Industrial grade iron hydroxide can serve as a suitable source of Fe(III) The results show that almost all dissolved phosphate is recovered by the reaction with Fe2+. Additionally, the activity of iron-reducing bacteria inhibits the production of sulphide by sulphate-reducing bacteria and the growth of these bacteria in anaerobic digesters.Документ Effect of iron hydroxide on phosphate removal during anaerobic digestion of activated sludge(2004) Stabnikov, Victor; Tay, Stephen; Tay, Joo; Ivanov, Volodymyr M.Addition of ferric hydroxide to the reactor of methanogenic fermentation of activated sludge followed microbial reduction of Fe (III) and formation of ferrous ions, which precipitated phosphate. It was shown that 66.6 – 99.6 % of dissolved phosphate with initial concentration of 1000 – 3500 mg РО43-/l can be removed by the addition of ferric hydroxide in concentration of 6420 mg Fe (III)/l and anaerobic sludge with iron-reducing activity. Optimal ratio of added Fe(III): removed dissolved phosphate, ensured not less than 95 % removal of phosphate was 2. These data could be used in a new technology of anaerobic treatment of wastewater with simultaneous removal of phosphate.Документ Application of iron-reducing bacteria for phosphate removal from returned liquor of municipal wastewater treatment plant(2005) Ivanov, Volodymyr M.; Stabnikov, Victor; Tay, Stephen; Tay, JooThe aim of this research was to examine efficiency of enrichment culture and isolated strains of iron-reducing bacteria for the removal of phosphate from return liquor of municipal wastewater treatment plant (MWWTP) with ferric hydroxide as a source of Fe (III). Bacterial reduction of ferric hydroxide enhanced phosphate removal from return liquor. The obtained data could be used for the design of a new biotechnology of anaerobic removal or recovery of phosphate from return liquor of MWWTP.Документ Phosphate removal from return liquor of municipal wastewater treatment plant using iron-reducing bacteria(2005) Ivanov, Volodymyr M.; Stabnikov, Victor; Zhuang, W. Q.; Tay, Joo; Tay, StephenThe application of iron-reducing bacteria (IRB) for phosphate removal from return liquor (liquid fraction after activated sludge digestion and anaerobic sludge dewatering) of municipal wastewater treatment plant was studied. Methods and Results: Enrichment culture and two pure cultures of IRB, Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK identified by 16S rRNA gene sequencing, were produced using return liquor of WWTP as carbon and energy source and iron hydroxide as oxidant. The final concentration of phosphate increased from 70 to 90 mg l-1 in control and decreased from 70 to 1 mg l-1 in experiment. The mass ratio of removed P to produced Fe(II) was 0.17 g P g-1 Fe(II). S. maltophilia BK showed the ability to reduce Fe(III) using such xenobiotics as diphenylamine, m-cresol, 2,4-dichlorphenol and p-phenylphenol as sole source of carbon under anaerobic conditions. Bacterial reduction of ferric hydroxide enhanced phosphate removal from return liquor. Significance and Impact of Study: An ability of facultative anaerobes Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK to reduce Fe(III) was shown. These bacteria can be used for anaerobic removal of phosphate and xenobiotics by bacterial reduction of ferric ions.Документ The effect of various iron hydroxide concentrations on the anaerobic fermentation of sulfate-containing model wastewater(2006) Stabnikov, Victor; Ivanov, Volodymyr M.Addition of ferric hydroxide and iron-reducing bacteria in anaerobic treatment of sulphate-containing wastewater reduced sulphate reduction and production of sulphide, increased removal of total organic carbon (TOC) and methane production. Influence of ferric addition on sulphate-containing wastewater treatment depended on Fe(III) dosage, which can be determined as a molar ratio of Fe(ІІІ)/SO42-. Concentration of sulphide constantly increased and consisted on 15 day 91 mg/l and 45 mg/l at ratio of Fe(ІІІ)/SO42- 0.06 and 0.5. However, no production of dissolved sulphide was observed at ratio Fe(ІІІ)/SO42- 1 and 2. Maximum rates of total organic carbon removal were 0.75, 1.15, 1.39, 1.55 g TOC/g of volatile suspended solids (VVS) per hour; rates of methane production were 0.039, 0.047, 0.064 and 0.069 ml/g VVS per hour; contents of methane in biogas were 25, 41, 55 and 62 vol. % at the ratios of Fe(ІІІ)/SO42- 0.06, 0.5, 1 and 2, respectively. These data could be used for the development of a new technology for anaerobic treatment of sulphate containing wastewater.Документ The removal of phosphorus from reject water of municipal wastewater treatment plant using iron ore(2009) Guo, Cheng Hong; Stabnikov, Victor; Kuang, Shengli; Ivanov, Volodymyr M.BACKGROUND: Reject water (return liquor) from dewatering of anaerobically digested activated sludge in municipal wastewater treatment plants contains from 10 to50%of the phosphorus load when being recycled to the aeration tank. Phosphorus removal from reject water could be an effective way to decrease phosphorus loads entering the aeration tank. An innovative approach involves the replacement of iron salts, which are commonly used for phosphorus removal, with ferrous ions produced by iron-reducing bacteria from iron ore. The aim of the research was to examine the feasibility of phosphorus removal from return liquor using bioreduction of iron ore.RESULTS: Ferrous production, phosphate and organic carbon removal rates were determined as a function of different iron ore particle sizes in batch experiments. Iron-reducing bacteria ensured the production of ferrous ions from iron ore up to concentrations of 550 mg L−1. The ferrous production rate was linearly dependent on the calculated specific surface area of the iron ore particles. The phosphorus concentration in the reject water was reduced by 90% during bioreduction of iron ore. The phosphorus removal rate did not depend on specific surface area of iron ore particles when the particle size of iron ore was smaller than 7 mm. The organic carbon removal rate did not seem to be dependent on iron ore particle size. CONCLUSION: Removal of phosphate using iron ore can be more economical than conventional chemical precipitation of phosphate using iron salts because of the lower cost of iron ore.Документ The removal of phosphate from wastewater using anoxic reduction of iron ore in the rotating reactor(2009) Guo, Cheng Hong; Stabnikov, Victor; Kuang, Shengli; Ivanov, Volodymyr M.The removal of phosphorus from reject water, which is the liquid fraction produced after dewatering of anaerobically digested activated sludge on the municipal wastewater treatment plants (MWWTPs), can significantly reduce the phosphorus load to the main stream of the MWWTPs. Ferric or ferrous reagents can be used for this removal but the significantly cheaper option could be the production of ferrous reagent using bioreduction of iron ore. The removal of phosphorus from reject water using anoxic bioreduction of iron ore was studied in the rotating reactor, which was selected to avoid the clogging of the pores between iron ore particles. The highest phosphorus removal rate from reject water in the rotating reactor, i.e. the parameter which can be used in the design of the continuous process, was 25mg P/L day. Significant role in the iron ore bioreduction is playing the formation of the fine particles from initially loaded coarse particles of iron ore during rotation of the reactor.Документ The removal of nitrogen and phosphorus from reject water of municipal wastewater treatment plant using ferric and nitrate bioreductions(2010) Guo, Cheng Hong; Stabnikov, Victor; Ivanov, Volodymyr M.Reject water, which is the liquid fraction produced after dewatering of anaerobically digested activated sludge on the municipal wastewater treatment plants (MWWTPs), contributes up to 80% of the nitrogen and phosphorus loads to the MWWTP. It was proposed to combine the removal of nitrogen from reject water using the sequential biooxidation of and bioreduction of NO3- with precipitation of phosphate by Fe2+ ions produced due to bioreduction of Fe3+ in iron ore. Bioreduction of NO3- decreased Fe3+ bioreduction rate in reject water from 37 to 21 mg Fe2+/L d due to competition between NO3- and Fe3+ for electron donors. Addition of acetate as electron donor increased both bioreduction rates of Fe3+ and NO3- but acetate interfered with the competition between nitrate and phosphate anions reacting with ferrous cations decreasing efficiency of the phosphate removal from reject water. The stages of denitrification and ferric bioreduction/phosphate precipitation must be performed sequentially.