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Документ Impact of microbial Nocardia vaccinii IMB B-7405 surfactants on oil destruction in water(2016) Pirog, Tatiana; Panasiuk, Catherine; Antoniuk, NinaThe degree of oil degradation, as well as complex oil pollution with heavy metal in water in the presence of Nocardia vaccinii ІMV B-7405 biosurfactants was studied. The destruction of crude oil (2,6−6,0 g/l) in water) at 25−30 days after treatment with the post-fermentative culture liquid (5−10% v/v) containing biosurfactant was 76−94%.Документ Microbial surfactants in environmental technologies(2015) Pirog, Tatiana; Konon, Anastasia; Savenko, IngaIn the review the literature and own experimental data on the use of microbial surface active glycolipids (rhamno-, sophoro- and trehalose lipids) and lipopeptides for water and soil purification from oil and other hydrocarbons, removing toxic heavy metals (Cu2+, Cd2+, Ni2+, Pb2+), degradation of complex pollution (oil and other hydrocarbons with heavy metals), and the role of microbial surfactants in phytoremediation processes was shown. The factors that limit the use of microbial surfactants in environmental technologies are discussed. Thus, at certain concentrations biosurfactant can exhibit antimicrobial properties and inhibit microorganisms destructing xenobiotics. Microbial biodegradability of surfactants may also reduce the effectiveness of bioremediation.Документ Improvement of the technology for surfactant synthesis by Аcinetobacter calcoaceticus ІМV В-7241(2015) Pirog, Tatiana; Konon, AnastasiaThe aim of the work was to intensify the synthesis of surfactants by Acinetobacter calcoaceticus IMV-7241 cultivated on ethanol and other carbon substrates. A. calcoaceticus IMV-7241 was grown in the medium with mono- (ethanol, glycerol, liquid paraffin, n-hexadecane, glucose) and mixed substrates in the presence of organic acids or heavy metal cations (0.1–2.0 mM Cu2+, Cd2+, Zn2+, Pb2+). The synthesis of surfactants was evaluated by emulsification index of cultural liquid, conditional concentration and concentrations of extracellular surfactants, which were determined gravimetrically after their extraction from supernatant with the mixture of methanol and chloroform. It was shown that addition of citrate and fumarate (0.01%) at the end of exponential growth phase of A. calcoaceticus ІМV В-7241 in the medium with ethanol (2%) and the maintenance of neutral pH increased the surfactants’ concentration in 3.5 times (up to 6.0 g/l). The quantity of extracellular surfactants synthesized by the strain ІМV В-7241 in the medium containing mixture of n-hexadecane and glycerol (molar ratio 1:7) and C/N 30 was increased in 2.6–3.5 times in comparison with cultivation on corresponding monosubstrates. Addition of 2.0 mM Cu2+ at the stationary growth phase of A. calcoaceticus ІМV В-7241 in medium with liquid paraffin and n-hexadecane led to the increase of surfactants’ synthesis in 2.3–2.5 times compared with those in the medium without Cu2+. Approaches to intensification of surfactants’ synthesis by A. calcoaceticus ІМV В-7241 (including addition of biosynthesis precursors and cultivation on the mixture of substrates) can be used to increase the efficiency of microbial technologies.