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Документ Bacteriocins Lactobacillus – an alternative to antimicrobial drugs(2021) Voloshina, Iryna; Soloshenko, Kateryna; Krasinko, Victoria; Lych (Tkachenko), Inna; Shkotova, LyudmilaThe review presents the characteristics of bacteria of the Lactobacillus family and their ability to synthesize various bacteriocins. The classification of lactobacilli bacteriocins is given, which includes three classes: class I – lantibiotics (peptides with a molecular weight of less than 5 kDa, which containing lanthionine), class II – unmodified bacteriocins, also called non-lantibiotics (heat-resistant peptides, which do not contain lanthionine and have a molecular weight of less 10 kDa) and class III – a poorly studied group of thermolabile proteins with a molecular weight of more than 30 kDa. Lactobacilli are shown to synthesize a wide spectrum of bacteriocins, which demonstrate a variety of actions and are able to inhibit the growth of numerous species of opportunistic gram-positive microflora. The article also provides the examples of lactobacilli bacteriocins isolated from food products (fermented meat, fish, kombucha, goat milk, koumiss, etc.) and various human biotopes (microbiota of breast milk, intestinal tract, and vaginal secretions). Additionally, the review shows the prospects of wide application of bacteriocins synthesized by lactobacilli in the food and pharmaceutical industries.Документ Determination of molecular weights of biologically active fragments of colostrum by electrophoresis method(2017) Lych (Tkachenko), Inna; Veriovka, Serhii; Voloshina, IrynaIt has been determined that high molecular weight fractions of colostrum contain protein molecules with molecular weights of 160-190 kDa, among which are immunoglobulins. Separate parts of immunoglobulins and other proteins, namely, IgG (55kDa) and IgA (62kDa) heavy chains, secretory component of sIgA, and lactoferrin, have also been found out. The test samples of high molecular weight peptides of colostrum contain the biologically active fragments among which there are mostly immunoglobulins, their parts, proteinsabzymes, other protein fragments. The proteins with the molecular weight of 80, 62, 55 kDa are also determined, they can represent heavy chains (55 kDa) and light chains (25 kDa) of IgG and sIgA. In particular, sIgA is a supermolecular complex consisting of Н- and L-chains of IgA (62, 25 kDa, respectively) and a secretory component (80 kDa). Apart from the secretory component of IgA, the protein molecules with the molecular weight of 80 kDa can be halves of the molecules of immunoglobulins or lactoferrin.Документ Practical use of goat milk and colostrum(2021) Voloshina, Iryna; Soloshenko, Kateryna; Lych (Tkachenko), Inna; Shkotova, LyudmilaThis review presents the protein and amino acid composition of both goat colostrums and milk and describes the properties of goat colostrums and milk components. In addition, the prospects of use of goat milk and colostrum in the food and cosmetics industry and the feasibility of use of goat milk for baby feeding are shown. Functional foods produced from goat milk have antioxidant, anti-inflammatory, cardioprotective, antihypertensive and antiatherogenic activities in the human body. Goat milk cosmetics are very useful for maintaining a healthy skin and are effective in treatment of various skin diseases. Infant formula based on goat milk provides comfortable digestion for babies and are better at absorbing proteins, fats and other nutrients than infant formula based on cow’s milk.Документ Polyfunctional properties of goat colostrum proteins and their use(2020) Soloshenko, Kateryna; Lych (Tkachenko), Inna; Voloshina, Iryna; Shkotova, LyudmilaThe review presents the composition of goat colostrum, antimicrobial, immunomodulatory and antioxidant properties of biologically active proteins of goat colostrum, as well as the prospects of their use in medicine. Due to the presence of a complex of biologically active proteins such as lactoferrin, lysozyme, lactoperoxidase, immunoglobulins, etc., goat colostrum increases the body’s resistance to infectious diseases, strengthens the immune system, has an antioxidant effect and antibacterial activity against a wide range of microorganisms, thereby preventing or delaying chronic diseases associated with the oxidative stress. Additionally, goat colostrum bioactive proteins show antitumor activity, antiatherogenic properties, the ability to lower the blood pressure and efficiency in the treatment of rheumatoid arthritis.Документ Enzyme lysozyme: methods for production and perspectives(2012) Karevina, Victoria; Voloshina, IrynaThe methods for the production of enzyme lysozyme from animal tissues, plant biomass and microorganisms are described. The recombinant bacterial strains of Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum were constructed for production of lysozyme. It was shown the construction of recombinant strains of Lactobacillus plantarum and Lactobacillus fermentum producing lysozyme lysing cells of staphylococci. These strains could be used in the composition of new probiotics.Документ The prospects of using bacteria of the genus Rhodococcus and microbial surfactants for the degradation of oil pollutants(2006) Vildanova-Marcishin, R.; Karpenko, Elena; Shcheglova, N.; Pirog, Tatiana; Voloshina, IrynaThe possibility of accelerating oil degradation by an enrichment culture of oil-oxidizing microorganisms in the presence of bacteria of the genus Rhodococcus and microbial surfactants was studied. It was shown that the degree of consumption of crude oil (2vol %) after 192 h of enrichment culture growth reached 84%. Inoculation of the active hydrocarbon-oxidizing strain Rhodococcus erythropolis EK-1 and exogenous surfactants produced by Pseudomonas sp. PS-27 increased this degree to 90 and 93–94%, respectively. On the grounds of these results, efficient methods of purification of the environment from oil pollutants can be developed.Документ Production of Surfactants by Rhodococcus erythropolis Strain EK-1, Grown on Hydrophilic and Hydrophobic Substrates(2004) Pirog, Tatiana; Shevchuk, Tetiana; Voloshina, Iryna; Karpenko, ElenaThe ability of Rhodococcus erythropolis strain EK-1 to produce surfactants when grown on hydrophilic (ethanol and glucose) and hydrophobic (liquid paraffins and hexadecane) substrates was studied. The strain was found to produce surfactants with emulsifying and surface-active properties. The production of surfactants depended on the composition of the nutritive medium, nature and concentration of the sources of carbon and nitrogen, and duration of cultivation. Chemically, surfactants produced by Rhodococcus erythropolis EK-1 grown on ethanol are a complex of lipids with polysaccharide–proteinaceous substances. The lipids include glycolipids (trehalose mono- and dicorynomycolates) and common lipids (cetyl alcohol, palmitic acid, methyl n-pentadecanoate, triglycerides, and mycolic acids).Документ Use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil(2005) Gregirchak, Natalia; Voloshina, Iryna; Pirog, Tatiana; Shevchuk, TetianaOil-oxidizing bacteria were isolated from oil-polluted soil and water samples and identified as Acinetobacter calcoaceticus K-4, Nocardia vacсinii K-8, Rhodococcus erythropolis EK-1, and Mycobacterium sp. K-2. It was found that immobilization of the bacteria on an expanded clay aggregate accelerated their growth and consumption of hydrocarbon substrates. It was also found that water polluted with 100 mg/l oil could be purified with Rhodococcus erythropolis EK-1 and Nocardia vacсinii K-8 cells immobilized in this way. The dependence of the degree of water purification on its flow rate, aeration, and availability of nitrogen and phosphorus sources was determined. The efficiency of water purification from oil by immobilized Rhodococcus erythropolis EK-1 cells at high flow rates (of up to 0.68 l/h), low aeration (of 0.1 l/l per min) and an intermittent supply of 0.01% diammonium phosphate reached 99.5–99.8%.