Pirog, TatianaKuzminska, Yu.2012-04-022012-04-022003Pirog, T. Regulation of Acetate Metabolism in a Strain of Acinetobacter sp. Growing on Ethanol / T. Pirog, Yu. Kuzminska // Applied Biochemistry and Microbiology. - 2003. - Vol. 39, No. 2. - P. 158-165.https://dspace.nuft.edu.ua/handle/123456789/667Ethanol metabolism in Acinetobacter sp. is shown to be limited by the rate of acetate assimilation,a reaction catalyzed by acetyl-CoA synthetase (EC 6.2.1.1). Effects of ions (sodium, potassium, and magnesium), by-products of ethanol and acetaldehyde oxidation (NADH and NADPH), and pantothenic acid on this enzyme are studied (sodium, NADH, and NADPH inhibit acetyl-CoA synthetase; pantothenic acid, potassium, and magnesium act as enzyme activators). Conditions of culturing were developed under which ethanol, acetaldehyde, and acetate in Acinetobacter cells were oxidized at the same rates, producing a threefold increase in the activity of acetyl-CoA synthetase in the cell-free extract. The results of studies of acetyl-CoA synthetase regulation in a mutant strain of Acinetobacter sp., which is incapable of forming exopolysaccharides, provide a basis for refining the technology of ethapolan production involving the use of C2 substrates.en-USethanol metabolismметаболизм этанолаacetyl-CoA synthetaseregulationexopolysaccharides synthesisацетил-КоА-синтетазарегуляциясинтез экзополисахаридовметаболізм етанолуацетил-КоА-синтетазарегуляціясинтез екзополісахаридівкафедра біотехнології і мікробіологіїArticle