Pirog, TatianaKarpenko, ElenaAntoniuk, SvitlanaShevchuk, Tetiana2012-04-052012-04-052009The influence of culture conditions strain Acinetobacter calcoaceticus K-4 on the synthesis of surfactant/ T. Pirog, S. Antonyuk, Ye. Karpenko, T. Shevchuk // Applied Biochemistry and Microbiology - 2009 - Vol. 45, No. 3 - P. 272–278.https://dspace.nuft.edu.ua/handle/123456789/733It has been observed that the Acinetobacter calcoaceticus K-4 strain produces surface-active substances (SAS) while growing either on hydrophilic (ethanol) or on hydrophobic substrates (hexadecane). Maximal SAS synthesis (with a conditional SAS concentration of 3.6; emulsifying activity of culture liquid dissolved in 50 times equal to 96%) was detected with growth on an ethanol-containing medium with the addition of urea, yeast autolysate and microelements, C/N ratio 60:1 and 10% inoculate, cultivated on ethanol-containing medium by the end of the exponential phase of growth. With respect to its chemical nature, extracellular SAS synthesized by A. calcoaceticus K-4 growing on ethanol-containing medium under optimal cultivating conditions form a glycolipid–aminolipid complex.en-USbiosurfactantsповерхностно-активные веществаethanolcondition cultivationповерхнево-активні речовиниетанолумови культивуванняэтанолусловия культивированиякафедра біотехнології і мікробіологіїArticle