Intensification of Surfactant Synthesis in Rhodococcus erythropolis EK-1 Cultivated on Hexadecane

dc.contributor.authorPirog, Tatianaen_EN
dc.contributor.authorKlimenko, Juliaru_RU
dc.contributor.authorShevchuk, Tetianaen_EN
dc.date.accessioned2012-04-05T09:59:27Z
dc.date.available2012-04-05T09:59:27Z
dc.date.issued2010
dc.description.abstractActivity of key enzymes of n-alkane metabolism was determined in cells of Rhodococcus erythropolis EK-1, a surfactant producer grown on n hexadecane. Potassium cations were found to inhibit alkane hydroxylase and NADP+-dependent aldehyde dehydrogenase, while sodium cations were found to activate these enzymes. Decreased potassium concentration (to 1 mM), increased sodium concentration (to 35 mM), and addition of 36 μmol/l Fe(II), required for alkane hydroxylase activity, resulted in increased activity of the enzymes of n-hexadecane metabolism and in a fourfold increase of surfactant synthesis. A 1.5–1.7-fold increase in surfactant concentration after addition of 0.2% fumarate (gluconeogenesis precursor) and 0.1% citrate (lipid synthesis regulator) to the medium with hexadecane results from enhanced synthesis of trehalose mycolates, as evidenced by a 3–5- fold increase in phosphoenolpyruvate synthetase and trehalose phosphate synthase, respectively.В клетках штамма-продуцента поверхностно-активных веществ (ПАВ) Rhodococcus erythropolis ЭК-1, выращенного на н-гексадекане, определена активность ключевых ферментов метаболизма н-алканов.uk_UK
dc.identifier.citationPirog, T. Intensification of Surfactant Synthesis in Rhodococcus erythropolis EK-1 Cultivated on Hexadecane / T. Pirog, Yu. Klimenko, T. Shevchuk // Applied Biochemistry and Microbiology. − 2010. − Vol. 46, № 6. − P. 599–606.en_EN
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/734
dc.language.isoen_USuk_UK
dc.subjectintensificationen_EN
dc.subjectrhodococcus erythropolisen_EN
dc.subjectn-alkane metabolismen_EN
dc.subjectsurfactanten_EN
dc.subjectrhodococcus erythropolisru_RU
dc.subjectінтенсифікаціяuk_UK
dc.subjectrhodococcus erythropolisru_RU
dc.subjectметаболізм н-алканівuk_UK
dc.subjectповерхнево-активні речовиниuk_UK
dc.subjectкафедра біотехнології і мікробіології
dc.titleIntensification of Surfactant Synthesis in Rhodococcus erythropolis EK-1 Cultivated on Hexadecaneuk_UK
dc.typeArticleuk_UK

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