Effect of hydrolytic enzymes pretreatment on the oil extraction from pumpkin seeds

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dc.contributor.authorNosenko, Tamara
dc.contributor.authorVovk, Ganna
dc.contributor.authorKorolyuk, Tamara
dc.date.accessioned2019-06-10T07:58:16Z
dc.date.available2019-06-10T07:58:16Z
dc.date.issued2019
dc.description.abstractIntroduction. Enzyme assisted technologies were proposed for oil seeds processing. Currently there are no reports about pumpkin seeds oil recovering using enzyme assisted pretreatment. The aim of the present study was to evaluate the effect of enzyme mixture on the cell integrity, oil yield of cold-pressing and dynamics of solvent extraction of pumpkin seeds oil. Materials and Methods. Protolad, Alkaline, acid proteases and Cellulad (ENZIME, Ukraine) were used for pumpkin seeds pretreatment. The cells integrity was evaluated by the method of immediate "shaking". The cold pressing was carried out on the laboratory screw press. The solvent oil extraction rate was estimated using a Soxhlet extractor as oil quantity extracted after one extraction cycle. Results and discussion. It was detected that main increase of pumpkin seed cells integrity destruction comparing with a control sample had been happened after 2-hour of enzyme pretreatment. Further incubation of ground seeds with enzymes did not lead to significant increase of "open" cells content in the mixture. It was shown that using of different kind of proteolitic enzymes for pumpkin seeds pretreatment resulted in increase of destroyed cells quantity from 3 to 10.4 %. Using of proteases and cellulase mixture for pumpkin seeds pretreatment had resulted in further increase of level of pumpkin seed cells "revealing" by 10 %. The oil yield of cold pressed pumpkin oil after enzyme pretreatment with protease (70 %) and cellulase (30 %) mixture was increased from 62.3 (control sample) to 70.0 % from total oil content of seeds. The rate of solvent extraction of oil from pumpkin seeds had increased after enzyme pretreatment, that means 25.4 and 17.7 % of oil were extracted after 80 min extraction from mass of enzyme pretreated and control seeds, respectively. There was no difference of peroxide content between enzyme pretreated sample and control. Conclusions. Using of proteases and cellulases mixture for pumpkin seeds pretreatment had resulted in increase of destroyed cells quantity, following by increase of cold pressed pumpkin oil as well as rate of solvent extraction of oil from pumpkin seeds.uk_UA
dc.identifier.citationNosenko, T. Effect of hydrolytic enzymes pretreatment on the oil extraction from pumpkin seeds / T. Nosenko, G. Vovk, T. Koroluk // Ukrainian Food Journal. – 2019. – Vol. 8, Issue (1). – P. 80–88.uk_UA
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/29603
dc.language.isoenuk_UA
dc.subjectкафедра технології жирів, хімічних технологій харчових добавок та косметичних засобівuk_UA
dc.subjectsolvent extractionuk_UA
dc.subjectpumpkin seedsuk_UA
dc.subjectoiluk_UA
dc.subjectproteaseuk_UA
dc.subjectcellulaseuk_UA
dc.subjectpressinguk_UA
dc.subjectнасіння гарбузівuk_UA
dc.subjectоліяuk_UA
dc.subjectпротеазаuk_UA
dc.subjectцелюлазаuk_UA
dc.subjectхолодне пресуванняuk_UA
dc.subjectекстрагування розчинникомuk_UA
dc.subjectсемена тыквыuk_UA
dc.subjectмаслоuk_UA
dc.subjectпротеазаuk_UA
dc.subjectцеллюлазаuk_UA
dc.subjectхолодное прессованиеuk_UA
dc.subjectэкстрагирование растворителемuk_UA
dc.titleEffect of hydrolytic enzymes pretreatment on the oil extraction from pumpkin seedsuk_UA
dc.typeArticleuk_UA

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