The influence of culture conditions on the physico-chemical properties of exopolysaccharide etapolana

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dc.contributor.authorPirog, Tatianaen_EN
dc.contributor.authorKorzh, Yuliyaru_RU
dc.contributor.authorShevchuk, Tetianaen_EN
dc.date.accessioned2012-04-03T07:06:59Z
dc.date.available2012-04-03T07:06:59Z
dc.date.issued2009
dc.description.abstractThe physicochemical properties of the complex exopolysaccharide ethapolan (EPS) produced by Acinetobacter sp. 12S during growth on media with various C/N ratios and different concentrations of mineral components and phosphate buffer were studied. Irrespective of the cultivation conditions, the concentrations of carbohydrates (38–44%) and pyruvic acid (3.2–3.7%) in the total EPS, as well as in the acylated (AP) and nonacylated (NAP) polysaccharides obtained from them, were practically the same. The EPS, AP, and NAP were also identical in their monosaccharide composition: the molar ratio of glucose, mannose, galactose, and rhamnose was 3 : 2 : 1 : 1. The polysaccharides contained different concentrations of mineral salts (6–28%), uronic acid (3.7–22.0%), and fatty acids (5.8–15.4%); they also differed in the ratio of acetylated and nonacetylated polysaccharides. Due to the differences in the chemical composition and molecular mass (500 kDa – 1.5 MDa), the viscosities of the EPS solutions (in the presence of 0.1 M KCl, in the H+–form, and in Cu2+–glycine system) were different as well. The mechanisms responsible for changes in the physicochemical properties of the total EPS, AP, and NAP synthesized on various media are discussed.uk_UK
dc.identifier.citationPirog, T. Rheological properties of the microbial exopolysaccharide ethapolan syntesized in the presence of exogenous fatty acids in the culture medium / T. Pirog, Yu. Korzh, T. Shevchuk // Applied Biochemistry and Microbiology - 2012. - Vol. 45, No. 1 - P. 50–55.
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/677
dc.language.isoen_USuk_UK
dc.subjectexopolysaccharideuk_UK
dc.subjectэкзополисахаридыuk_UK
dc.subjectacylated and nonacylated polysaccharides
dc.subjectphysicochemical properties
dc.subjectchemical composition
dc.subjectmolecular mass
dc.subjectcultivation сonditions
dc.subjectацилированный и неацилированный полисахариды
dc.subjectфизико-химические свойства
dc.subjectхимический состав
dc.subjectмолекулярная масса
dc.subjectусловия культивирования
dc.subjectекзополісахариди
dc.subjectацілірованная і неацілірованний полісахариди
dc.subjectфізико-хімічні властивості
dc.subjectхімічний склад
dc.subjectмолекулярна маса
dc.subjectумови культивування
dc.subjectкафедра біотехнології і мікробіології
dc.titleThe influence of culture conditions on the physico-chemical properties of exopolysaccharide etapolanauk_UK
dc.typeArticleuk_UK

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