Exopolysaccharide Production and Peculiarities of C6-Metabolism in Acinetobacter sp. Grown on Carbohydrate Substrates

dc.contributor.authorPirog, Tatianaen_EN
dc.contributor.authorKovalenko, M.en_EN
dc.contributor.authorKuzminska, Yu.en_EN
dc.date.accessioned2012-04-03T06:27:54Z
dc.date.available2012-04-03T06:27:54Z
dc.date.issued2002
dc.description.abstractAn Acinetobacter sp. strain grown on carbohydrate substrates (mono- and disaccharides, molasses, starch) was shown to synthesize exopolysaccharides (EPS). Glucose catabolism proved to proceed via the Embden–Meyerhof–Parnas and Entner–Doudoroff pathways. Pyruvate entered the tricarboxylic acid cycle due to pyruvate dehydrogenase activity. Pyruvate carboxylation by pyruvate carboxylase was the anaplerotic reaction providing for the synthesis of intermediates for the constructive metabolism of Acinetobacter sp. grown on C6-substrates. The C6-metabolism in Acinetobacter sp. was limited by coenzyme A. Irrespective of the carbohydrate growth substrate (glucose, ethanol), the activities of the key enzymes of both C2- and C6-metabolism was high, except for the isocitrate lyase activity in glucose-grown bacteria. Isocitrate lyase activity was induced by C2-compounds (ethanol or acetate). After their addition to glucose-containing medium, both substrates were utilized simultaneously, and an increase was observed in the EPS synthesis, as well as in the EPS yield relative to biomass. The mechanisms responsible for enhancing the EPS synthesis in Acinetobacter sp. grown on a mixture of C2- and C6-substrates are discussed.uk_UK
dc.identifier.citationPirog, T. Exopolysaccharide Production and Peculiarities of C6-Metabolism in Acinetobacter sp. Grown on Carbohydrate Substrates / T. Pirog, M. Kovalenko, Yu. Kuzminska // Microbiology. – 2002. – Vol. 71, №. 2. – P. 182–188.en_EN
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/675
dc.language.isoen_USuk_UK
dc.subjectexopolysaccharidesuk_UK
dc.subjectэкзополисахаридыuk_UK
dc.subjectglucose metabolismen_EN
dc.subjectregulationen_EN
dc.subjectmixed substratesen_EN
dc.subjectintensification of biosynthesisen_EN
dc.subjectметаболизм глюкозыru_RU
dc.subjectрегуляцияru_RU
dc.subjectсмешанные субстратыru_RU
dc.subjectинтенсификация биосинтезаru_RU
dc.subjectекзополісахаридиuk_UK
dc.subjectметаболізм глюкозиuk_UK
dc.subjectрегуляціяuk_UK
dc.subjectзмішані субстратиuk_UK
dc.subjectінтенсифікація біосинтезуuk_UK
dc.subjectкафедра біотехнології і мікробіології
dc.titleExopolysaccharide Production and Peculiarities of C6-Metabolism in Acinetobacter sp. Grown on Carbohydrate Substratesuk_UK
dc.typeArticleuk_UK

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