Regulation of Acetate Metabolism in a Strain of Acinetobacter sp. Growing on Ethanol
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Дата
2003
Автори
ORCID
DOI
Науковий ступінь
Рівень дисертації
Шифр та назва спеціальності
Рада захисту
Установа захисту
Науковий керівник
Члени комітету
Видавець
Анотація
Ethanol metabolism in Acinetobacter sp. is shown to be limited by the rate of acetate assimilation,a reaction catalyzed by acetyl-CoA synthetase (EC 6.2.1.1). Effects of ions (sodium, potassium, and magnesium), by-products of ethanol and acetaldehyde oxidation (NADH and NADPH), and pantothenic acid on this enzyme are studied (sodium, NADH, and NADPH inhibit acetyl-CoA synthetase; pantothenic acid, potassium, and magnesium act as enzyme activators). Conditions of culturing were developed under which ethanol, acetaldehyde, and acetate in Acinetobacter cells were oxidized at the same rates, producing a threefold increase in the activity of acetyl-CoA synthetase in the cell-free extract. The results of studies of acetyl-CoA synthetase regulation in a mutant strain of Acinetobacter sp., which is incapable of forming exopolysaccharides, provide a basis for refining the technology of ethapolan production involving the use of C2 substrates.
Опис
Ключові слова
ethanol metabolism, метаболизм этанола, acetyl-CoA synthetase, regulation, exopolysaccharides synthesis, ацетил-КоА-синтетаза, регуляция, синтез экзополисахаридов, метаболізм етанолу, ацетил-КоА-синтетаза, регуляція, синтез екзополісахаридів, кафедра біотехнології і мікробіології
Бібліографічний опис
Pirog, T. Regulation of Acetate Metabolism in a Strain of Acinetobacter sp. Growing on Ethanol / T. Pirog, Yu. Kuzminska // Applied Biochemistry and Microbiology. - 2003. - Vol. 39, No. 2. - P. 158-165.