Some characteristics of central metabolism in Acinetobacter sp. grown on ethanol

Скорочена інформація про документ
dc.contributor.authorPirog, Tatianaen_EN
dc.contributor.authorKuzminska, Yu.en_EN
dc.date.accessioned2012-04-02T13:31:02Z
dc.date.available2012-04-02T13:31:02Z
dc.date.issued2003
dc.description.abstractEthanol-grown cells of the mutant Acinetobacter sp. strain 1NG, incapable of producing exopolysaccharides, were analyzed for the activity of enzymes of the tricarboxylic acid (TCA) cycle and some biosynthetic pathways. In spite of the presence of both key enzymes (isocitrate lyase and malate synthase) of the glyoxylate cycle, these cells also contained all enzymes of the TCA cycle, which presumably serves biosynthetic functions. This was evident from the high activity of isocitrate dehydrogenase and glutamate dehydrogenase and the low activity of 2-oxoglutarate dehydrogenase. Pyruvate was formed in the reaction catalyzed by oxaloacetate decarboxylase, whereas phosphoenolpyruvate (PEP) was synthesized by the two key enzymes (PEP carboxykinase and PEP synthase) of gluconeogenesis. The ratio of these enzymes was different in the exponential and the stationary growth phases. The addition of the C4-dicarboxylic acid fumarate to the ethanolcontaining growth medium led to a 1.5- to 2-fold increase in the activity of enzymes of the glyoxylate cycle, as well as of fumarate hydratase, malate dehydrogenase, PEP synthase, and PEP carboxykinase (the activity of the latter enzyme increased by more than 7.5 times). The data obtained can be used to improve the biotechnology of production of microbial exopolysaccharide ethapolan on C2-substrates.uk_UK
dc.identifier.citationPirog, T. Some characteristics of central metabolism in Acinetobacter sp. grown on ethanol / T. Pirog, Yu. Kuzminska // Microbiology. - 2003. - Vol. 72, No. 4. - P. 408-413.en_EN
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/671
dc.language.isoen_USuk_UK
dc.subjectethanol metabolismuk_UK
dc.subjectметаболизм этанолаuk_UK
dc.subjecttricarboxylic acid cycleen_EN
dc.subjectglyoxylate cycleen_EN
dc.subjectgluconeogenesisen_EN
dc.subjectbiosynthesisen_EN
dc.subjectцикл трикарбоновых кислотru_RU
dc.subjectглиоксилатный циклru_RU
dc.subjectглюконеогенезru_RU
dc.subjectбиосинтезru_RU
dc.subjectметаболізм етанолуuk_UK
dc.subjectцикл трикарбонових кислотuk_UK
dc.subjectгліоксилатний циклuk_UK
dc.subjectглюконеогенезuk_UK
dc.subjectбіосинтезuk_UK
dc.subjectкафедра біотехнології і мікробіології
dc.titleSome characteristics of central metabolism in Acinetobacter sp. grown on ethanoluk_UK
dc.typeArticleuk_UK

Файли

Контейнер файлів
Зараз показуємо 1 - 1 з 1
Ескіз
Назва:
MCRO_2003_1.pdf
Розмір:
41.69 KB
Формат:
Adobe Portable Document Format
Завантажити
Ліцензійна угода
Зараз показуємо 1 - 1 з 1
Ескіз недоступний
Назва:
license.txt
Розмір:
1.71 KB
Формат:
Item-specific license agreed upon to submission
Опис:
Завантажити

Зібрання

Статті