Flow cytometric analysis of Ca2+-induced changes of membrane potential in smooth muscles mitochondria
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A flow cytometric protocol for the analysis of membrane potential and Са2+ level in isolated myometrium mitochondria was developed. Ca2+ addition induced the mitochondrial membrane depolarization. At the presence of Mg2+ and ATP, the Ca2+ addition did not cause the membrane potential dissipation. It was demonstrated that the flow cytometry is a useful tool to analyze Са2+ level and membrane potential in isolated myometrium mitochondria.
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Flow cytometric analysis of Са2+-induced changes of membrane potential in smooth muscles mitochondria / L. Babich, S. Shlykov, N. Naumova (Kandaurova), S. Kosterin // Annales Universitatis Mariae Curie-Sklodowska. Lublin - Polonia. Sectio DDD. – 2008. – Vol. XXI, N 2, 69. – P. 353-356.