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Application of surface-active substances produced by Rhodococcus erythropolis IMB Aс-5017 for post-harvest treatment of sweet cherry
(2022) Pirog, Tatiana; Stabnikov, Victor; Antoniuk, Svitlana
Introduction. The aim of the present study was testing of the supernatant of Rhodococcus erythropolis ІМВ Ас-5017 with different concentration of surface-active substances (SAS) for treatment of sweet cherry for shelf-life extension. Materials and methods. R. erythropolis ІМВ Ас-5017 were grown in the medium with ethanol. Supernatant with concentration of SAS from 0.1 to 0.5 g/L was used for the treatment of sweet cherry fruit. Concentration of SAS in supernatant was determined by weight method. The total number of heterotrophic bacteria and fungi were determined by the plate dilution method. Results and discussion. The treatment of sweet cherries with a supernatant containing 0.5 g/L SAS diminished the numbers of bacteria and fungi on the fruit’s surface by 10 and 5 times, respectively, in comparison with cherries washed with water. The treatment of sweet cherries with supernatant containing 0.2 g/L SAS diminished the numbers of bacteria and fungi on the fruit’s surface by 5 and 3 times, respectively; treatment with supernatant containing 0.1 g/L diminished the numbers of bacteria and fungi by 2 times in comparison with cherries washed with water. The treatment with supernatant with concentration SAS 0.5 g/L was most effective. Treated with supernatant sweet cherries fruits did not show signs of decay even on 7th day of storage, while untreated or washed with water fruits lost moisture, fruit’s skin became wrinkled, cracks and decayed areas appeared on it. Content of fungal cells on the surface of sweet cherry pretreated with supernatant with concentration of SAS from 0.1 to 0.5 g/L and after that contaminated with spore’s suspension of Aspergillus niger Р-3 were by 2 – 11 times lower than on the surface of fruits washed with water after 5 days of incubation. The possibility of multiple usage of supernatant was shown. Application of supernatant with concentration of 0.5 g/L resulted in decrease of bacterial concentration after first usage by 10 times, after second usage it was diminished by 5 times and after third usage it was diminished by 3 times, meanwhile concentration of fungi decreased by 9, 5 and 4 times after I, II, and III usage of supernatant. Conclusion. Surface-active substances synthesized by Rhodococcus erythropolis IMB Ac-5017 could be used for treatment of sweet cherry to extend their shelf life.
Biosurfactant of acinetobacter calcoaceticus імv в–7241: influence of Cu2+ on synthesis and use in bioremediation processes
(2012) Andrushchenko, Yaroslav; Konon, Anastasia; Antoniuk, Svitlana; Pirog, Tatiana; Parfenyuk, Sergey
The influence of Cu2+ on surfactant synthesis by Acinetobacter calcoaceticus IMV B-7241 and possibility of their using for the degradation of complex oil pollution of soil and water was studied
Degradation of aromatic compounds by the oil-oxidizing bacteria Nocardia vacciniiк-8 and Acenitobacter calcoaceticus ІМВ В-7241
(2012) Antoniuk, Svitlana
Nowadays the problem of environmental remediation of aromatic xenobiotics is rather actual because of their pronounced carcinogenic and mutagenic properties. Biotechnologies may be an alternative to physical and chemical methods of environmental remediation thanks for the high destructive potential of the microorganism, ecological safety and cheapness. It was shown that the oil-oxidizing bacteria Nocardia vaccinii К-8 та Acenitobacter calcoaceticus ІМВ В-7241 assimilated substrates of aromatic nature and synthesized practically valuable surfactants. The growth of strains K-8 and IMV B-7241 on aromatic compounds was intensified in 1.5-2 times in the case of the consecutive cultivation of the inoculum of the studied strains on liquid medium containing aromatic compounds (0.1-0.25%) compared to using the inoculum cultivated on the meat infusion agar. These strains are promising for use in the remediation of water and soil polluted with aromatic xenobiotics.
Industrial waste bioconversion into surfactants by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii IMV B-7405
(2017) Pirog, Tatiana; Shulyakova, Mariya; Kliuchka (Nykytyuk), Lilia; Antoniuk, Svitlana; Elperin, Igor
The aim of the work is to realize an alternative processing of toxic industrial waste into surfactants by strains Rhodacoccus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii IMV B-7405 for remediation of environment. The studied strains were grown in liquid media containing such sources of carbon as waste (fried) sunflower oil. technical glycerol (by product of biodiesel production), and aromatic compounds. The synthesis of surfactants was evaluated by emulsification index. conditional concentration of surfactants and concentration of extracellular surfactants, which was determined gravimetrically after their extraction from supernatant by the mixture of methanol and chloroform. The concentration of oil in water and soil was analyzed by gravimetric method after extaction with hexane.
Influence of medium pH on surfactant synthesis by acinetobacter calcoaceticus IMV B-7241 on ethanol
(2012) Konon, Anastasia; Antoniuk, Svitlana; Chebotaryova, Ksenia; Parfenyuk, Sergey; Pirog, Tatiana
It was stated that during the cultivation of Acinetobacter calcoaceticus IMV B-7241 in the medium with ethanol the pH maintenance at 6–7 was accompanied by the 2-fold increase of the concentration of synthesized metabolites with surface-active properties. Under such conditions of cultivation the qualitative composition of the synthesized neutral, phospho- and glycolipids was almost unchanged compared with the cultivation without pH regulation.
Influence of medium ph on surfactant synthesis by Acinetobacter calcoaceticus IMV B-7241 on hydrophilic substrate ethanol
(2012) Antoniuk, Svitlana; Konon, Anastasia; Chebotaryova, Ksenia
The synthesis of exocellular metabolites with surface-active and emulsifying properties was investigated at the maintaining pH at 5.0-8.0 rate during the cultivation of Acinetobacter calcoaceticus IMV B-7241 on the medium with ethanol (2% by volume). It was stated that the maintaining pH at 7.0 by the KOH solution was accompanied by the increase of surfactant synthesis in 1.8 times comparing with the process without pH regulation. The replacement of KOH on NaOH for the maintaining pH at an optimal level led to the decrease of surfactant concentration in 1.2-1.5 times that was caused by the inhibitory effect of sodium cations on the activity of enzymes of surface-active amino- and glycolipids biosynthesis.
Nocardia vaccinii К-8 and acenitobacter calcoaceticus ІМV В-7241 – potential destructors of aromatic compounds
(2012) Antoniuk, Svitlana; Sofіlkanich (Morozova), Anna; Pirog, Tatiana
It is shown that Nocardia vaccinii K-8 and Acenitobacter calcoaceticus IMV B-7241 are able to assimilate aromatic compounds, as well as tosynthesize practically valuable surfactants. That’s why these strains are promisingfor usein the remediation of water and soil polluted with crude oilandaromatic xenobiotics. Показано, що Nocardia vaccinii К-8 та Acenitobacter calcoaceticus ІМВ В-7241 здатні не тільки асимілювати ароматичні сполуки, а й синтезувати при цьому практично цінні поверхнево-активні речовини, завдяки чому є перспективними для використання в очищенні довкілля від комплексних забруднень як нафтопродуктами, так і ксенобіотиками ароматичної природи.
The influence of culture conditions strain Acinetobacter calcoaceticus K-4 on the synthesis of surfactant
(2009) Pirog, Tatiana; Karpenko, Elena; Antoniuk, Svitlana; Shevchuk, Tetiana
It has been observed that the Acinetobacter calcoaceticus K-4 strain produces surface-active substances (SAS) while growing either on hydrophilic (ethanol) or on hydrophobic substrates (hexadecane). Maximal SAS synthesis (with a conditional SAS concentration of 3.6; emulsifying activity of culture liquid dissolved in 50 times equal to 96%) was detected with growth on an ethanol-containing medium with the addition of urea, yeast autolysate and microelements, C/N ratio 60:1 and 10% inoculate, cultivated on ethanol-containing medium by the end of the exponential phase of growth. With respect to its chemical nature, extracellular SAS synthesized by A. calcoaceticus K-4 growing on ethanol-containing medium under optimal cultivating conditions form a glycolipid–aminolipid complex.
The properties of surfactants synthesized by Acinetobacter calcoaceticus ІMV В-7241 on refined and sunflower oil waste
(2018) Pirog, Tatiana; Lutsay, Dariya; Antoniuk, Svitlana; Elperin, Igor
The aim of the work was to compare the antimicrobial and anti-adhesive activity (including the ability to destroy biofilms), as well as the effect on oil degradation of the surfactants synthesized by the culture of Acinetobacter calcoaceticus IMV B-7241 on refined and sunflower waste. The microbial surfactants were extracted from supernatant of cultural liquid by mixture of chloro form and methanol (2:1). The number of attached cells and the degree of biofilm destruction were analyzed spectrophotometrically. Antimicrobial properties of the surfactants were determined by index of the minimal inhibiting concentration. The concentration of oil in water was analyzed by the gravimetric method after extraction with hexane. It was shown that microbial surfactants synthesized in medium with 2% of both refined and fried oil were characterized by high antimicrobial (MIC with respect to bacterial test cultures 0.8–29 μg/ml, Candida albicans D-6 26−58 μg/ml) and anti-adhesive (decreasing number of bacterial and fungal cells of test cultures attached to abiotic surfaces by 35–70%, destruction of biofilms by an average of 40–44%) activity. Increasing concentration of waste oil in the medium to 4% was accompanied by the formation of surfactants with low antimicrobial activity, in the presence of which the degree of oil destruction in water (3–6 g/l) was 80–88% in 20 days, which is 10–16% higher than when using surfactants synthesized in a medium with 2% oil. Obtained data indicate on the need for studies on the effect of cultivation conditions of producer on the properties of synthesized surfactants for the production of final product with stable predetermined properties, depending on the field of practical application. Метою роботи було порівняти антимікробну та антиадгезійну активність (у тому числі здатність руйнувати біоплівки), а також вплив на деградацію олії ПАР, синтезованих культурою Acinetobacter calcoaceticus ІМВ В-7241 на рафінаді та соняшнику. відходи. Мікробні поверхнево-активні речовини екстрагували із супернатанту культуральної рідини сумішшю хлороформи та метанолу (2:1). Кількість прикріплених клітин і ступінь руйнування біоплівки аналізували спектрофотометрично. Антимікробні властивості ПАР визначали за показником мінімальної інгібуючої концентрації. Концентрацію олії у воді аналізували гравіметричним методом після екстракції гексаном. Показано, що мікробні ПАР, синтезовані на середовищі з 2% як рафінованої, так і смаженої олії, характеризуються високою антимікробною дією (МІК щодо бактеріальних тест-культур 0,8–29 мкг/мл, Candida albicans D-6 26–58 мкг/мл). антиадгезивну (зменшення кількості бактеріальних і грибкових клітин тест-культур, прикріплених до абіотичних поверхонь, на 35–70%, руйнування біоплівок у середньому на 40–44%). Підвищення концентрації відпрацьованої нафти в середовищі до 4 % супроводжувалося утворенням поверхнево-активних речовин із низькою антимікробною активністю, у присутності яких ступінь деструкції нафти у воді (3–6 г/л) у 20 р. становив 80–88 %. днів, що на 10–16 % вище, ніж при використанні ПАР, синтезованих у середовищі з 2 % олії. Отримані дані свідчать про необхідність дослідження впливу умов культивування продуцента на властивості синтезованих ПАР для отримання кінцевого продукту зі стабільними заданими властивостями в залежності від галузі практичного застосування.