Перегляд за Автор "Shulyakova, Mariya"

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Biosurfactants of Rhodococcus erythropolis IMV Ас-5017: synthesis intensification and practical application
(2013) Pirog, Tatiana; Sofіlkanich (Morozova), Anna; Shulyakova, Mariya; Shevchuk, Tetiana
Intensification of the surfactant synthesis by Rhodococcus erythropolis IMV Ac-5017 on different substrates, including industrial waste, as well as the use of surfactant preparations for oil degradation were studied. It was established that the addition of fumarate (0.2 %) and citrate (0.1 %) into the medium with ethanol, n-hexadecane, or glycerol (1–2 %) was accompanied by an increase of conditional surfactant concentration by 1.5–1.7 times compared to the indexes in the medium without organic acids. The intensification of surfactant synthesis in the presence of fumarate and citrate is caused by the increased activity of isocitrate lyase (by 1.2–15-fold) and enzymes of the surfactant biosynthesis (by 2–4.8-fold) compared to their activity in the medium without precursors. The possibility of surfactant synthesis intensification (by 3–4-fold) while cultivating of R. erythropolis IMV Ac-5017 in the medium with oil containing substrates (2 %) and glucose (0.1 %) was shown. The introduction of 0.01 mM Cu2+ in the exponential growth phase of strain IMV Ac-5017 in the medium with ethanol accompanied by the increasing conditional surfactant concentration by 1.9 times. The highly efficient remediation (92–95 %) of oil (2–2.6 g/L) and Cu2+ polluted water after treatment with surfactant preparations (native cultural liquid) at low concentrations (5 %) was determined.
Glycerol metabolism in producers of surface-active substances rhodococcus erythropolis імv ас-5017, acinetobacter calcoaceticus імv в-7241 and nocardia vaccinіi к-8
(2012) Mashenko, Oksana; Shulyakova, Mariya; Pirog, Tatiana; Shevchuk, Tetiana
It was established that the strains Rhodococcus erythropolis ІМВ Ас-5017, Acinetobacter calcoaceticus ІМВ В-7241 and Nocardia vaccinіi К-8 metabolize glycerol to dihydroxyacetonephosphate via two known pathways (glycerol-3-phosphate pathway and dihydroxyacetone pathway). The data obtained could be the basis for theoretical calculations of the energy needs for surfactants and biomass synthesis on this substrate to determine the optimal molar ratio of energy unequivalent substrates in order to increase the synthesis of surfactants of R. erythropolis ІМV Ас-5017, A. calcoaceticus IMV B-7241 and N. vaccinii K-8 on the mixture of substrates.
Industrial waste bioconversion into surfactants by Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii IMV B-7405
(2017) Pirog, Tatiana; Shulyakova, Mariya; Kliuchka (Nykytyuk), Lilia; Antoniuk, Svitlana; Elperin, Igor
The aim of the work is to realize an alternative processing of toxic industrial waste into surfactants by strains Rhodacoccus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMV B-7241 and Nocardia vaccinii IMV B-7405 for remediation of environment. The studied strains were grown in liquid media containing such sources of carbon as waste (fried) sunflower oil. technical glycerol (by product of biodiesel production), and aromatic compounds. The synthesis of surfactants was evaluated by emulsification index. conditional concentration of surfactants and concentration of extracellular surfactants, which was determined gravimetrically after their extraction from supernatant by the mixture of methanol and chloroform. The concentration of oil in water and soil was analyzed by gravimetric method after extaction with hexane.
Intensification of surface-active substances synthesis of Rhodococcus Erythropolis IMV Ac-5017 and Acinetobacter Calcoaceticus IMV B-7241 on glycerol
(2012) Pirog, Tatiana; Shevchuk, Tetiana; Shulyakova, Mariya; Mashenko, Oksana; Konon, Anastasia
Proposed approaches such as use of glycerol as a cheap substrate, adding of exogenous precursors of biosynthesis, using a mixture of growth substrates, may be the basis for the development of economically beneficial industrial technologies of producing of surfactants of Acinetobacter calcoaceticus IMV B-7241 and Rhodococcus erythropolis IMV Ac-5017 and help to solve the problem of disposal of waste of biodiesel production. Запропоновані підходи, а саме використання гліцерину як дешевого субстрату, внесення екзогенних попередників біосинтезу, використання суміші ростових субстратів, можуть стати основою для розробки економічно вигідної промислової технології отримання поверхнево-активних речовин Acinetobacter calcoaceticus ІМВ В-7241 та Rhodococcus erythropolis ІМВ Ас-5017, а також вирішити проблему утилізації відходів виробництва біодизелю.