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Документ Environmental belief of students in higher technical educational institutions – the path to harmonious relationship between technogenic society and nature(2011) Skrotska, Oksana; Voloshina, Iryna; Krasinko, Victoria; Lych (Tkachenko), Inna; Teterina, SvitlanaThe article is devoted to the consideration of ways of the topical problems decision which get in way of harmonization of relations between technogenic society and the nature. It also devoted to the analysis of state of training of specialists on ecological orientation in the higher technical educational institutions.Документ Enzyme lysozyme: methods for production and perspectives(2012) Karevina, Victoria; Voloshina, IrynaThe methods for the production of enzyme lysozyme from animal tissues, plant biomass and microorganisms are described. The recombinant bacterial strains of Staphylococcus aureus, Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum were constructed for production of lysozyme. It was shown the construction of recombinant strains of Lactobacillus plantarum and Lactobacillus fermentum producing lysozyme lysing cells of staphylococci. These strains could be used in the composition of new probiotics.Документ Polyfunctional properties of goat colostrum proteins and their use(2020) Soloshenko, Kateryna; Lych (Tkachenko), Inna; Voloshina, Iryna; Shkotova, LyudmilaThe review presents the composition of goat colostrum, antimicrobial, immunomodulatory and antioxidant properties of biologically active proteins of goat colostrum, as well as the prospects of their use in medicine. Due to the presence of a complex of biologically active proteins such as lactoferrin, lysozyme, lactoperoxidase, immunoglobulins, etc., goat colostrum increases the body’s resistance to infectious diseases, strengthens the immune system, has an antioxidant effect and antibacterial activity against a wide range of microorganisms, thereby preventing or delaying chronic diseases associated with the oxidative stress. Additionally, goat colostrum bioactive proteins show antitumor activity, antiatherogenic properties, the ability to lower the blood pressure and efficiency in the treatment of rheumatoid arthritis.Документ Production of Surfactants by Rhodococcus erythropolis Strain EK-1, Grown on Hydrophilic and Hydrophobic Substrates(2004) Pirog, Tatiana; Shevchuk, Tetiana; Voloshina, Iryna; Karpenko, ElenaThe ability of Rhodococcus erythropolis strain EK-1 to produce surfactants when grown on hydrophilic (ethanol and glucose) and hydrophobic (liquid paraffins and hexadecane) substrates was studied. The strain was found to produce surfactants with emulsifying and surface-active properties. The production of surfactants depended on the composition of the nutritive medium, nature and concentration of the sources of carbon and nitrogen, and duration of cultivation. Chemically, surfactants produced by Rhodococcus erythropolis EK-1 grown on ethanol are a complex of lipids with polysaccharide–proteinaceous substances. The lipids include glycolipids (trehalose mono- and dicorynomycolates) and common lipids (cetyl alcohol, palmitic acid, methyl n-pentadecanoate, triglycerides, and mycolic acids).Документ The prospects of using bacteria of the genus Rhodococcus and microbial surfactants for the degradation of oil pollutants(2006) Vildanova-Marcishin, R.; Karpenko, Elena; Shcheglova, N.; Pirog, Tatiana; Voloshina, IrynaThe possibility of accelerating oil degradation by an enrichment culture of oil-oxidizing microorganisms in the presence of bacteria of the genus Rhodococcus and microbial surfactants was studied. It was shown that the degree of consumption of crude oil (2vol %) after 192 h of enrichment culture growth reached 84%. Inoculation of the active hydrocarbon-oxidizing strain Rhodococcus erythropolis EK-1 and exogenous surfactants produced by Pseudomonas sp. PS-27 increased this degree to 90 and 93–94%, respectively. On the grounds of these results, efficient methods of purification of the environment from oil pollutants can be developed.Документ Use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil(2005) Gregirchak, Natalia; Voloshina, Iryna; Pirog, Tatiana; Shevchuk, TetianaOil-oxidizing bacteria were isolated from oil-polluted soil and water samples and identified as Acinetobacter calcoaceticus K-4, Nocardia vacсinii K-8, Rhodococcus erythropolis EK-1, and Mycobacterium sp. K-2. It was found that immobilization of the bacteria on an expanded clay aggregate accelerated their growth and consumption of hydrocarbon substrates. It was also found that water polluted with 100 mg/l oil could be purified with Rhodococcus erythropolis EK-1 and Nocardia vacсinii K-8 cells immobilized in this way. The dependence of the degree of water purification on its flow rate, aeration, and availability of nitrogen and phosphorus sources was determined. The efficiency of water purification from oil by immobilized Rhodococcus erythropolis EK-1 cells at high flow rates (of up to 0.68 l/h), low aeration (of 0.1 l/l per min) and an intermittent supply of 0.01% diammonium phosphate reached 99.5–99.8%.