Effect of cations on the activity of NADP+-dependent glutamate dehydrogenase in Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017 and Nocardia vaccinii IMV B-7405 grown on industrial waste

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dc.contributor.authorPirog, Tatiana
dc.contributor.authorPaliichuk, Olesia
dc.contributor.authorLutsay, Dariya
dc.contributor.authorKliuchka (Nykytyuk), Lilia
dc.contributor.authorShevchuk, Tetiana
dc.date.accessioned2023-09-19T08:23:12Z
dc.date.available2023-09-19T08:23:12Z
dc.date.issued2021
dc.description.abstractIntroduction. It is studied the activity of NADP+-dependent glutamate dehydrogenase in the presence of mono- and divalent cations (potential activators of this key enzyme of surface-active aminolipids biosynthesis) in A. calcoaceticus IMV B-7241, R. erythropolis IMV Ac-5017 and N. vaccinii IMV B-7405 during cultivation on waste of biodiesel production and sunflower oil waste. Materials and methods. Cultivation of strains was performed in liquid mineral media using as substrates: refined and waste (after frying potato) sunflower oil, refined glycerol and waste of biodiesel production. NADP+-dependent (EC 1.4.1.4) glutamate dehydrogenase activity in cell-free extracts was analyzed for glutamate formation during oxidation of NADPH at 340 nm. Monovalent (Na+, K+) and divalent (Mg2+, Ca2+, Zn2+) cations in the form of salts of NaCl, KCl, MgSO4 × 7H2O, CaCl2 and ZnSO4 × 7H2O were added to the reaction mixture, as well as into the medium for strains cultivation. Results and discussion. Calcium cations were found to be activators of NADP+-dependent glutamate dehydrogenase activity in R. erythropolis IMV Aс-5017 and N. vaccinii IMV B-7405 grown on refined and waste sunflower oil: in the presence of 1–5 mmol Ca2+ in the mixture, the activity of the enzyme increased 1.3–2 times compared with that without these cations. The increase in the concentration of CaCl2 to 0.2−0.4 g/l in oil-containing medium of strains IMV Ac-5017 and IMV B-7405 cultivation was accompanied by an increase in NADP+-dependent glutamate dehydrogenase activity by 1.3–1.5 times compared with that on basic medium. When additional quantity of CaCl2 (0.1−0.2 g/l) was introduced into the medium with purified glycerol for the cultivation of A. calcoaceticus IMV B-7241, an increase in NADP+-dependent glutamate dehydrogenase activity was observed by almost 2.5−3 times compared with those for strain IMV B-7241 on the basic medium. There was no impact of activating cations magnesium, zinc, potassium and sodium on NADP+-dependent glutamate dehydrogenase activity of all strains grown on oil-containing substrates and glycerol of different degrees of purification. Conclusion. The results demonstrate the possibility to increase activity of key enzymes of the biosynthesis of the desired product: the composition of the medium should be modified by changing the content of enzymes’ activators.uk_UA
dc.identifier.citationEffect of cations on the activity of NADP+-dependent glutamate dehydrogenase in Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017 and Nocardia vaccinii IMV B-7405 grown on industrial waste / T. Pirog, O. Paliichuk, D. Lutsai, L. Klyuchka, T. Shevchuk // Ukrainian Food Journal. – 2021. – Vol. 10, Is. 1. − Pp. 198-208uk_UA
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/41083
dc.language.isoenuk_UA
dc.subjectглутаматдегідрогеназаuk_UA
dc.subjectактиваториuk_UA
dc.subjectвідходиuk_UA
dc.subjectповерхнево-активні речовиниuk_UA
dc.subjectкафедра біотехнології і мікробіологіїuk_UA
dc.subjectglutamate dehydrogenaseuk_UA
dc.subjectactivatorsuk_UA
dc.subjectwasteuk_UA
dc.subjectbiosurfactantsuk_UA
dc.titleEffect of cations on the activity of NADP+-dependent glutamate dehydrogenase in Acinetobacter calcoaceticus IMV B-7241, Rhodococcus erythropolis IMV Ac-5017 and Nocardia vaccinii IMV B-7405 grown on industrial wasteuk_UA
dc.typeArticleuk_UA

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