Calcium and magnesium cations influence on antimicrobial and antiadhesive activity of Acinetobacter сalcoaceticus ІMV B-7241 surfactants

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dc.contributor.authorPirog, Tatiana
dc.contributor.authorSidor, Inga
dc.contributor.authorLutsay, Dariya
dc.date.accessioned2018-01-15T09:56:11Z
dc.date.available2018-01-15T09:56:11Z
dc.date.issued2016
dc.description.abstractThe aim of the work was to study the effect of calcium and magnesium cations on NADP+-dependent glutamate dehydrogenase activity (key enzyme of biosynthesis of Acinetobacter calcoaceticus ІMV B-7241 surface-active aminolipids) followed by modification of medium composition and determining antimicrobial and antiadhesive activity of synthesized surfactants. The strain IMV B-7241 was grown in medium with ethanol. NADP+-dependent glutamate dehydrogenase activity of the cell-free extract was analyzed using the formation of glutamate in the oxidation of NADPH. Surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). Antimicrobial against bacteria properties of the surfactants were determined by index of the minimal inhibitory concentration. The number of attached cells and the degree of biofilm destruction were analyzed spectrophotometrically. It was established that in the presence of 10 mM Cа2+ and Mg2+ NADP+-dependent glutamate dehydrogenase activity in the cell-free extract increased to 1.5 times in comparison with that without cations. Increasing concentration of magnesium sulfate to 0.2 g/l, or adding CaCl2 (0.1 g/l) into cultivation medium of IMV B-7241 strain was accompanied by rise of NADP+-dependent glutamate dehydrogenase activity in 2.4 and 3.0 times respectively, as well as increasing antimicrobial and antiadhesive activity of synthesized surfactants. Minimal inhibitory concentration of surfactants synthesized in modified media against some bacteria was in 1.3–3.5 times, adhesion on abiotic surfaces treated with such surfactants in an average of 5–17% lower, and the degree of biofilm destruction in 7–13% higher as compared to indicators for the surfactant produced in the base medium. The obtained results indicate the possibility of regulating antimicrobial and anti-adhesive activity of surfactants under producer cultivation.uk_UA
dc.identifier.citationPirog, T. Calcium and magnesium cations influence on antimicrobial and antiadhesive activity of Acinetobacter сalcoaceticus ІMV B-7241 surfactants / T. Pirog, I. Sidor, D. Lutsai // Biotechnologia Acta. − 2016. − V. 9, N 6. − Р. 50−57.uk_UA
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/26521
dc.subjectsurfactantsuk_UA
dc.subjectactivity of NADP+-dependent glutamateuk_UA
dc.subjectdehydrogenaseuk_UA
dc.subjectcalcium and magnesium cationsuk_UA
dc.subjectповерхнево-активні речовиниuk_UA
dc.subjectНАДФ+-залежна глутаматдегідрогеназна активністьuk_UA
dc.subjectкатіони кальцію і магніюuk_UA
dc.subjectкафедра біотехнології і мікробіологіїuk_UA
dc.titleCalcium and magnesium cations influence on antimicrobial and antiadhesive activity of Acinetobacter сalcoaceticus ІMV B-7241 surfactantsuk_UA
dc.typeArticleuk_UA

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