Specific features of the synthesis of the exopolysaccharide ethapolan on a mixture of energy-deficient growth substrates

dc.contributor.authorPirog, Tatianaen_EN
dc.contributor.authorVysyatetska, Nadezhdaru_RU
dc.contributor.authorKorzh, Yuliyaru_RU
dc.date.accessioned2012-03-30T07:54:59Z
dc.date.available2012-03-30T07:54:59Z
dc.date.issued2007
dc.description.abstractIntensification of the synthesis of the microbial exopolysaccharide ethapolan by Acinetobacter sp. B-7005 was shown to occur on a mixture of energy-deficient growth substrates (acetate + glucose). When the bacterium grew on the substrate mixture, both substrates were utilized simultaneously; acetate was taken up by means of active transport at the expense of the energy of the proton-motive force. When acetate was present in the form of a sodium salt, the activities of acetyl-CoA synthetase and phosphoenolpyruvate synthetase (the key enzyme of gluconeogenesis) were tenfold higher than in the presence of potassium acetate, and the indexes of ethapolan synthesis were two times higher. The positive effect of Na+ on ethapolan synthesis is supposed to consist in the creation of ion gradients on the membrane, necessary for the generation of the proton-motive force. Simultaneous functioning of the glyoxylate cycle and pyruvate carboxylase reaction, as well as an increase in the activity of isocitrate lyase, malate synthase, and phosphoenolpyruvate synthetase, provide evidence of increased gluconeogenesis in the presence of the acetate + glucose mixture (as compared to gluconeogenesis on the corresponding monosubstrates).uk_UK
dc.identifier.citationPirog, T. Specific features of the synthesis of the exopolysaccharide ethapolan on a mixture of energy-deficient growth substrates / T. Pirog, N. Vysyatetska, Yu. Korzh // Microbiology. - 2007. - Vol. 76, No. 1. - P. 25-30.en_EN
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/622
dc.language.isoen_USuk_UK
dc.subjectenergy-deficient substrateuk_UK
dc.subjectэнергетически дефицитный субстратuk_UK
dc.subjectintensification of biosynthesisen_EN
dc.subjectexopolysaccharidesen_EN
dc.subjectgluconeogenesisen_EN
dc.subjectactive transporten_EN
dc.subjectинтенсификация биосинтезаru_RU
dc.subjectэкзополисахаридыru_RU
dc.subjectглюконеогенезru_RU
dc.subjectактивный транспортru_RU
dc.subjectенергетично дефіцитний субстратuk_UK
dc.subjectінтенсифікація біосинтезуuk_UK
dc.subjectекзополісахаридиuk_UK
dc.subjectглюконеогенезuk_UK
dc.subjectактивний транспортuk_UK
dc.subjectкафедра біотехнології і мікробіології
dc.titleSpecific features of the synthesis of the exopolysaccharide ethapolan on a mixture of energy-deficient growth substratesuk_UK
dc.typeArticleuk_UK

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