Constitutive expression of recombinant clumping factor of Staphylococcus aureus in Escherichia coli

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dc.contributor.authorIvanova, Victoria
dc.contributor.authorPozur, V.
dc.date.accessioned2013-01-15T08:23:20Z
dc.date.available2013-01-15T08:23:20Z
dc.date.issued2003
dc.description.abstractExperiments for optimization of the clfA gene expression in E.coli BL21 (DE3)Gold cells were conducted. It has been shown the reducing of the strain efficiency and the elimination of the plasmid during the cultivation of a producer. Several methods for raising the amount of plasmid-containing cells and increasing the yield of recombinant protein were proposed. Bacterial growth medium was selected to make this process more efficient. In such conditions after IPTG induction of the recombinant cells we have got high level of a soluble protein (about 30 % of total cellular protein).uk_UK
dc.identifier.citationIvanova, V. Constitutive expression of recombinant clumping factor of Staphylococcus aureus in Escherichia coli / V. Ivanova, V. Pozur // National Taras Shevchenko University of Kiev. - 2003. - P. 34.en_EN
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/5315
dc.language.isoenuk_UK
dc.subjectclumping factoren_EN
dc.subjectexpressionen_EN
dc.subjectхлопьеобразующий факторru_RU
dc.subjectэкспрессияru_RU
dc.subjectпластівцевоутворюючий факторuk_UK
dc.subjectекспресіяuk_UK
dc.subjectкафедра експертизи харчових продуктів
dc.titleConstitutive expression of recombinant clumping factor of Staphylococcus aureus in Escherichia coliuk_UK
dc.typeThesisuk_UK

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