Synergism of antimicrobial and anti-adhesive activity of Nocardia vaccinii IMV B-7405 surfactants in a mixture with essential oils

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dc.contributor.authorPirog, Tatiana
dc.contributor.authorKliuchka (Nykytyuk), Lilia
dc.contributor.authorKliuchka, Igor
dc.contributor.authorShevchuk, Tetiana
dc.contributor.authorIutynska, Galyna
dc.date.accessioned2023-09-19T08:34:13Z
dc.date.available2023-09-19T08:34:13Z
dc.date.issued2020
dc.description.abstractAn increase in the antibiotic resistance of pathogenic microorganisms has stimulated the search for alternatives to antibiotics substances of natural origin, which are essential oils (EO) and non-toxic biodegradable microbial surfactants. Aim. To investigate the antimicrobial and anti-adhesive activity of a mixture of EO and surfactants of Nocardia vaccinii IMV B-7405 synthesized on various oil-containing media. Methods. N. vaccinii IMV B-7405 was grown in medium containing as carbon source refined sunflower oil, oil after frying french fried potatoes, potato wedges and meat. The surfactants were extracted from supernatant of cultural liquid by modified Folch mixture. The antimicrobial action of tea tree, cinnamon and lemongrass EO, surfactants and their mixtures was determined by index of the minimum inhibitory concentration (MIC). Synergistic effect of surfactants and EO was evaluated by indicator of fractional inhibitory concentration. The degree of bacteria and fungi biofilms destruction under the action of surfactants, EO and their mixtures was determined by spectrophotometric method. Results. It was found that N. vaccinii IMV B-7405 surfactants synthesized on all oil-containing substrates showed a synergistic antimicrobial and anti-adhesive activity with the investigated EO. MIC of a surfactants and EO mixture against bacteria (Bacillus subtilis BT-2 (spores), Escherichia coli IEM-1, Staphylococcus aureus BMS-1) and yeast (Candida albicans D-6, Candida utilis BVS-65 and Candida tropicalis RE-2) were 2–20 μg/ml and were significantly lower than each compound separately (156–625 and 8–80 μg/ml for EO and surfactants, respectively). The destruction of bacterial and yeast biofilms under the action of a mixture of surfactants (20–40 μg/ml) and EO (20–40 μg/ml) was 1.3–2.9 times higher compared with using of each component separately at similar concentrations. Conclusions. The data presented the possibility of using a mixture of EO and surfactants not only to reduce their MIC, but also as effective antimicrobial and anti-adhesive agents.uk_UA
dc.identifier.citationSynergism of antimicrobial and anti-adhesive activity of Nocardia vaccinii IMV B-7405 surfactants in a mixture with essential oils / T. P. Pirog, L. V. Kliuchka, I. V. Kliuchka, T. A. Shevchuk, G. O. Iutynska // Microbiological Journal. − 2020. − Vol. 82, N 4. − Рp. 31-40uk_UA
dc.identifier.urihttps://dspace.nuft.edu.ua/handle/123456789/41092
dc.language.isoenuk_UA
dc.subjectповерхнево-активні речовиниuk_UA
dc.subjectефірні оліїuk_UA
dc.subjectсинергічна діяuk_UA
dc.subjectантимікробна активністьuk_UA
dc.subjectруйнування біоплівокuk_UA
dc.subjectкафедра біотехнології і мікробіологіїuk_UA
dc.subjectNocardia vaccinii IMV B-7405uk_UA
dc.subjectsurfactantsuk_UA
dc.subjectessential oilsuk_UA
dc.subjectsynergistic actionuk_UA
dc.subjectantimicrobial activityuk_UA
dc.subjectdestruction of biofilmsuk_UA
dc.titleSynergism of antimicrobial and anti-adhesive activity of Nocardia vaccinii IMV B-7405 surfactants in a mixture with essential oilsuk_UA
dc.typeArticleuk_UA

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