Перегляд за Автор "Kyrylenko, Roman"

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Effect of nitrogen and mineral composition of the high-concentrated wort made from starch-containing raw materials on the cultivation of yeast
(2017) Shiyan, Peter; Mudrak, Tatiana; Kyrylenko, Roman; Kovalchuk, Svitlana
We theoretically substantiated and experimentally confirmed the feasibility of enriching highly-concentrated wort made from starch-containing raw materials with nitrogen and mineral nutrition for yeast cells. The influence of nitrogen nutrition, as amine and ammoniac nitrogen, as well as mineral nutrition as nanoparticles and ions of metals was investigated. It was established experimentally that in the course of cultivating the yeast on wort with a concentration of 20, 28 % DS, a necessary precondition is the adjustment of nitrogen nutrition through additional introduction of glycine to serve as amine nitrogen, the concentration of 0.7 g/dm3 of wort, and ammoniac nitrogen (to serve as carbamide) in the amount of 800 g/m3, which would make it possible to increase the concentration of yeast cells to 280−430 mln/cm3 in the process of culturing the yeast depending on the concentration of wort and the type of nitrogen nutrition, respectively. Based on results of the present research on the influence of mineral nutrition in the nano- and ionic form on the yeastgeneration process, it was determined that the largest positive effect on the synthesis of yeast cells was exerted by Zn and Mg and the mixtures of these metals, both in nano- and in ionic form, whose concentration was 1.2 mg/cm3 and 50 g/m3, respectively; in this case, the number of yeast cells ranged from 360 to 480 mln/cm3 depending on the concentration of wort and the type of mineral nutrition. We have proven the feasibility of cyclic use of mineral nutrition, both in ionic and in nano forms, when cultivating yeast, in order to obtain cells with high physiological-biochemical activity. The use of mineral and nitrogen nutrition allowed us not only to obtain a high concentration of yeast cells, but also reduce the duration of yeast-generation to 15–20 hours and ensure their high regenerative and physiological activity during fermentation of wort with high concentrations of dry substances. Теоретично обґрунтовано та експериментально підтверджено доцільність збагачення високонцентрованого сусла із крохмалевмісної сировини азотним та мінеральним живленням для дріжджових клітин. Досліджено вплив азотного живлення в якості амінного та аміачного азоту, а також мінерального живлення в якості наночастинок та іонів металів. Експериментально встановлено, що додавання азотного і мінерального живлення дозволяє отримати високу концентрацію дріжджових клітин, з високою фізіолого-біохімічною активністю, а також скоротити тривалість дріжджегенерування.
Investigation of the influence of nanoparticles of metals on fermenttation of wort of high concentrations
(2017) Kovalchuk, Svitlana; Shiyan, Peter; Mudrak, Tatiana; Kuts, Anatoly; Kyrylenko, Roman
There was theoretically grounded and experimentally proved the expedience of enriching high-concentrated wort of the starch-containing raw material with such additional source of mineral nutrition for yeast cells as nanoparticles of metals. There was studied the influence of nanoparticles of metals on hydrolysis of biopolymers of the raw material and biosynthesis of organic compounds at wort fermentation. It was experimentally established, that the most positive influence of biosynthetic properties of yeast cells is realized by zinc and magnesium. At using zinc and magnesium, yeast biomass and alcohol content in mashes increase. Best chemical-technological indices of mashes were received at adding nanoparticles of zinc and magnesium at the stage of batch dilution.There was experimentally studied the synthesis of volatile organic admixtures in marsh distillates depending on point of adding nanoparticles of metals. Теоретично обґрунтовано та експериментально підтверджено доцільність збагачення високонцентрованого сусла із крохмалевмісної сировини додатковим джерелом мінерального живлення для дріжджових клітин, в якості наночастинок металів. Досліджено вплив наночастинок металів на гідроліз біополімерів сировини та біосинтез органічних сполук при зброджуванні сусла. Експериментально встановлено, що найбільш позитивний вплив на біосинтетичні властивості дріжджових клітин має цинк та магній. При застосуванні цинку та магнію біомаса дріжджів та вміст спирту в бражках зростає. Найкращі хіміко-технологічні показники бражок отримані при додаванні наночастинок цинку та магнію на стадії розрідження замісу. Експериментально досліджено синтез летких органічних домішок в бражних дистилятах залежно від точки внесення наночастинок металів
Selection of the complex of enzyme preparation for the hydrolysis of the constituents of grain at the fermentation of the wort of high
(2018) Mudrak, Tatiana; Kuts, Anatoly; Kovalchuk, Svitlana; Kyrylenko, Roman; Bondar, Nikolai
In this work we have selected the optimal complex of enzyme preparations for hydrolysis of constituents of grain raw materials at fermentation of wort of high concentrations. In the selection of enzyme systems, their effect on the technical and chemical parameters of mature broods during fermentation of wort is investigated. For research, corn starch of 69.0% was used. Based on experimental studies, it was found that the use of a complex of enzyme preparations - amylolytic Amylex 4T, saccharifying - Diazim TGA, proteolytic (Alfalase AFP), cytolytic (Laminex 750) and complex complex AF β-glucanase and xylanase XL combinations of their concentrations contributed to the intensification of the fermentation process of the wort and increase the accumulation of the target product - ethanol by 0.8-1.4%, depending on the concentration of the wort. The highest amount of ethanol was accumulated at the maximum dosage of additional enzyme preparations.
The use of poliheksametyleneguanidine (PHMG) salts in alcohol production from starch containing raw materials
(2012) Bondar, Nikolai; Shiyan, Peter; Mudrak, Tatiana; Kyrylenko, Roman
Expediency of the use of poliheksamethyleneguanidine (PHMG) salts is reasonable as the antiseptic preparation «Polidez» in alcohol production from starch-containing raw materials. In working environment its influence on the course of microbiological and biochemical processes, quality of semiproducts and alcohol was investigated and efficiency of the preparation «Polidez» for inhibition of contaminating microflora growth was confirmed.