Перегляд за Автор "Stabnikov, Victor"

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Application of iron-reducing bacteria for phosphate removal from returned liquor of municipal wastewater treatment plant
(2005) Ivanov, Volodymyr M.; Stabnikov, Victor; Tay, Stephen; Tay, Joo
The aim of this research was to examine efficiency of enrichment culture and isolated strains of iron-reducing bacteria for the removal of phosphate from return liquor of municipal wastewater treatment plant (MWWTP) with ferric hydroxide as a source of Fe (III). Bacterial reduction of ferric hydroxide enhanced phosphate removal from return liquor. The obtained data could be used for the design of a new biotechnology of anaerobic removal or recovery of phosphate from return liquor of MWWTP.
Application of surface-active substances produced by Nocardia vaccinii ІМВ В-7405 for the treatment of vegetables
(2019) Pirog, Tatiana; Beregova (Pokora), Khrystyna; Geichenko, Bogdana; Stabnikov, Victor
Application of surface-active substances (SAS) produced by Nocardia vaccinii ІМV В-7405 for shelf live extension of vegetables was studied.Materials and methods. Organic vegetables such as tomato, cucumbers, and squashes were washed with the solution of SAS produced by N. vaccinii ІМV В-7405 with concentrations of 0.25 or 0.5 g/L. Microbiological analysis was done before the beginning of the vegetables storage. Evaluation of vegetable quality was conducted by viewing during time of the storage. Results and discussions. The results of our research showed the efficiency of the application of biosurfactant produced by Nocardia vaccinii ІМV В-7405 using industrial wastes for shelf life extension of vegetables. Results of visible observations as well as microbiological analysis showed that the treatment of vegetables with SAS solutions at the concentrations of 0.25 and 0.5 g/L was more effective than washing them with tap water. The total number of heterotrophic bacteria and fungi in the samples decreased after treatment of vegetables with SAS of N. vaccinii ІMV B-7405 by 16–34 and 3–14 times, respectively, meanwhile the washing of vegetables with tap water decreased total microbial number only by 2–2.5 times. It was shown that vegetables washed with water spoiled faster than those treated with SAS solution. The advantages of application of this biosurfactant for vegetables post-harvest treatment are that (1) it can be used at the lower by 2–6 times concentration in comparison with other reported in literature microbial SAS, and (2) it can be produced using industrial wastes that will reduce the cost of its production.Conclusion. Biosurfactant produced by Nocardia vaccinii ІМV В-7405 can be used for the treatment of vegetables to extent their shelf life. Досліджено застосування поверхнево-активних речовин (ПАР) Nocardia vaccinii ІМV В-7405 для подовження терміну зберігання овочів. Матеріали і методи. Органічні овочі, такі як помідори, огірки, кабачки, мили розчином ПАР виробництва N. vaccinii ІМВ В-7405 з концентрацією 0,25 або 0,5 г/л. Мікробіологічне дослідження проводили перед початком зберігання овочів. Оцінку якості овочів проводили шляхом огляду протягом часу зберігання. Результати та обговорення. Результати наших досліджень показали ефективність застосування біосурфактанту виробництва Nocardia vaccinii ІМВ В-7405 з використанням промислових відходів для подовження терміну зберігання овочів. Результати наочних спостережень, а також мікробіологічного аналізу показали, що обробка овочів розчинами ПАР у концентрації 0,25 і 0,5 г/л була більш ефективною, ніж миття їх водопровідною водою. Загальна кількість гетеротрофних бактерій та грибів у зразках зменшувалася після обробки овочів ПАР N. vaccinii ІМВ В-7405 у 16–34 та 3–14 разів відповідно, тоді як миття овочів водопровідною водою зменшувало загальну мікробну кількість. лише в 2–2,5 рази. Показано, що вимиті водою овочі псувалися швидше, ніж оброблені розчином ПАР. Переваги застосування цього біосурфактанту для післязбиральної обробки овочів полягають у тому, що (1) його можна використовувати в меншій у 2–6 разів концентрації порівняно з іншими мікробними ПАР, зареєстрованими в літературі, та (2) його можна отримувати з використанням промислових відходів, що здешевить його виробництво. Висновок. Біосурфактант Nocardia vaccinii ІМV В-7405 можна використовувати для обробки овочів з метою збільшення терміну їх зберігання.
Application of surface-active substances produced by Rhodococcus erythropolis IMB Aс-5017 for post-harvest treatment of sweet cherry
(2022) Pirog, Tatiana; Stabnikov, Victor; Antoniuk, Svitlana
Introduction. The aim of the present study was testing of the supernatant of Rhodococcus erythropolis ІМВ Ас-5017 with different concentration of surface-active substances (SAS) for treatment of sweet cherry for shelf-life extension. Materials and methods. R. erythropolis ІМВ Ас-5017 were grown in the medium with ethanol. Supernatant with concentration of SAS from 0.1 to 0.5 g/L was used for the treatment of sweet cherry fruit. Concentration of SAS in supernatant was determined by weight method. The total number of heterotrophic bacteria and fungi were determined by the plate dilution method. Results and discussion. The treatment of sweet cherries with a supernatant containing 0.5 g/L SAS diminished the numbers of bacteria and fungi on the fruit’s surface by 10 and 5 times, respectively, in comparison with cherries washed with water. The treatment of sweet cherries with supernatant containing 0.2 g/L SAS diminished the numbers of bacteria and fungi on the fruit’s surface by 5 and 3 times, respectively; treatment with supernatant containing 0.1 g/L diminished the numbers of bacteria and fungi by 2 times in comparison with cherries washed with water. The treatment with supernatant with concentration SAS 0.5 g/L was most effective. Treated with supernatant sweet cherries fruits did not show signs of decay even on 7th day of storage, while untreated or washed with water fruits lost moisture, fruit’s skin became wrinkled, cracks and decayed areas appeared on it. Content of fungal cells on the surface of sweet cherry pretreated with supernatant with concentration of SAS from 0.1 to 0.5 g/L and after that contaminated with spore’s suspension of Aspergillus niger Р-3 were by 2 – 11 times lower than on the surface of fruits washed with water after 5 days of incubation. The possibility of multiple usage of supernatant was shown. Application of supernatant with concentration of 0.5 g/L resulted in decrease of bacterial concentration after first usage by 10 times, after second usage it was diminished by 5 times and after third usage it was diminished by 3 times, meanwhile concentration of fungi decreased by 9, 5 and 4 times after I, II, and III usage of supernatant. Conclusion. Surface-active substances synthesized by Rhodococcus erythropolis IMB Ac-5017 could be used for treatment of sweet cherry to extend their shelf life.
Biocement: green building-and energy-saving material
(2012) Chu, Jian; Ivanov, Volodymyr M.; Stabnikov, Victor; He, Jia; Li, Bing; Naemi, Maryam
Cement and chemical grouts have often been used for soil strengthening. However, high cost, energy consumption, and harm to environment restrict their applications. Biocement could be a new green building- material and energy-saving material. Biocement is a mixture of enzymes or microbial biomass with inorganic chemicals, which can be produced from cheap raw materials. Supply of biocementing solution to the porous soil or mixing of dry biocement with clayey soil initiate biocementation of soil due to specific enzymatic activity. Different microorganisms and enzymes can be used for production of biocement.
Development of microbial geotechnology in Singapore
(2011) Chu, Jian; Ivanov, Volodymyr M.; He, Jia; Li, Bing; Naemi, Maryam; Stabnikov, Victor
Both nature processes and laboratory studies have shown that microorganisms can be used to improve the engineering properties of soil. As such, it is possible to develop methods that utilize the microbial process to treat soil in a way similar to that of cement. When more knowledge is accumulated through research findings and technology development, a new branch of geotechnical engineering – the Microbial Geotechnology can be established. The Microbial Geotechnology can have the following three applications: (a) biocementation to increase the strength of soil, (b) bioclogging to reduce the permeability of soil, and (c) biogas to increase the liquefaction resistance of sandy soil. Some types of microorganisms or bioprocesses that may contribute to the biocementation, bioclogging or biogas effects are identified and discussed. Some experimental data are presented to show that the permeability of sand can be reduced by four orders of magnitude and the strength of sand can be increased to a substantial value after the soil has been treated using bacteria. However, the whole study still stays at the laboratory stage and much more efforts are required to turn this scientific idea into viable technologies
Effect of complex baking improver on prolonging freshness of bakery products with reduced salt content
(2022) Bilyk, Olena; Stabnikov, Victor; Vasheka, Oksana; Bondarenko, Yuliia; Kochubei-Lytvynenko, Oksana
The aim of research was to determine the effect of a complex baking improver on prolonging the freshness of bakery products with a reduced salt content using the principles of lean manufacturing. Sociological research on the requirements for the quality of bakery products was conducted. A composition of a complex improver "Mineral Freshness Plus” was developed and its optimal dosage was determined. The effects of the developed improver addition on the quality of bakery products with reduced salt content of 0.5% by weight of flour, and the effect on the processes of staling products during storage were studied. According to sociological studies the presence of ingredients of synthetic origin in bakery products and rapid loss of their freshness is undesirable for consumers. To meet the consumer demands, the use of the principles of lean manufacturing in bakery production is provided, which consists in reducing technological costs and losses due to application of the developed improver. A recipe for a complex baking improver Mineral Freshness Plus has been developed, which is designed to intensify the technological process, improve consumer properties and time for keeping the freshness of bakery products with low salt content of 0.5%. The composition of the improver includes food additives with Generally Recognized as Safe status. Addition of "Mineral Freshness Plus" improver in quality of 2.0% to flour weight extends freshness of bakery products. This is confirmed by the higher by 65.2–75.6% content of dextrins in the product with the improver comparштп with the control, the thinner subcrustal layer on 72 hours of products storage, and changes in the structure of the bread pulp, which consists of interspersed swollen and partially gelatinized grains of starch, wrapped in a continuous mass of coagulated proteins. The use of the developed improver "Mineral Freshness Plus” in the manufacture of bakery products ensures the high consumer demands.
Effect of iron hydroxide on phosphate removal during anaerobic digestion of activated sludge
(2004) Stabnikov, Victor; Tay, Stephen; Tay, Joo; Ivanov, Volodymyr M.
Addition of ferric hydroxide to the reactor of methanogenic fermentation of activated sludge followed microbial reduction of Fe (III) and formation of ferrous ions, which precipitated phosphate. It was shown that 66.6 – 99.6 % of dissolved phosphate with initial concentration of 1000 – 3500 mg РО43-/l can be removed by the addition of ferric hydroxide in concentration of 6420 mg Fe (III)/l and anaerobic sludge with iron-reducing activity. Optimal ratio of added Fe(III): removed dissolved phosphate, ensured not less than 95 % removal of phosphate was 2. These data could be used in a new technology of anaerobic treatment of wastewater with simultaneous removal of phosphate.
Factors affecting antibiofilm properties of microbial surfactants
(2021) Pirog, Tatiana; Kliuchka (Nykytyuk), Lilia; Lutsay, Dariya; Stabnikov, Victor
Antibiofilm activity of surface-active substances (SAS) synthesized by Rhodococcus erythropolis, Acinetobacter calcoaceticus, or Nocardia vaccinia was studied. The strains were cultivated using carbon ethanol, glycerol, hexadecane, sunflower oil, and carbon-containing wastes from biodiesel production as carbon sources. Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, and yeasts Candida albicans and Candida utilis were used as testing cultures. The antibiofilm activity of biosurfactants depended on the source and concentration of carbon source and duration of cultivation. The addition of calcium cations to media significantly increased antibiofilm activity. Replacement of pure sources of carbon for wastes provided the opportunity to receive biosurfactants with high antibiofilm properties on inexpensive substrates. Destruction of biofilms was the same in cases when supernatant or purified SAS was used. Вивчено антибіоплівкову активність поверхнево-активних речовин (ПАР), синтезованих Rhodococcus erythropolis, Acinetobacter calcoaceticus або Nocardia vaccinia. Штами культивували з використанням в якості джерел вуглецю вуглецевого етанолу, гліцерину, гексадекану, соняшникової олії та вуглецевмісних відходів виробництва біодизеля. Як тест-культури використовували Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, дріжджі Candida albicans і Candida utilis. Антибіоплівкова активність біосурфактантів залежала від джерела та концентрації джерела вуглецю та тривалості культивування. Додавання до середовищ катіонів кальцію значно підвищувало активність антибіоплівки. Заміна чистих джерел вуглецю на відходи дала можливість отримувати на недорогих субстратах біоПАР з високими антибіоплівковими властивостями. Деструкція біоплівок була однаковою у випадках використання супернатанту або очищеного ПАР.
Formation of water-impermeable crust on sand surface using biocement
(2011) Stabnikov, Victor; Naemi, Maryam; Ivanov, Volodymyr M.; Chu, Jian
This paper examines the feasibility of using calcium-based biocement to form an impermeable crust on top of a sand layer. The biocement used was a mixture of calcium salt, urea, and bacterial suspension, which hydrolyzed urea with production of carbonate and an increase of the pH level. Applying 0.6 g of Ca per cm2 of sand surface, the permeability of the biocemented sand can be reduced from 10−4 m/s to 1.6•10−7 m/s (or 14 mm/day) due to formation of the crust on sand surface. The rupture modulus (maximum bending stress) of the crust was 35.9 MPa, which is comparable with that of limestone. The formation of a water-impermeable and high strength crust layer on sand surface could be useful for the construction of aquaculture ponds in sand, stabilization of the sand dunes, dust fixation in the desert areas, and sealing of the channels and reservoirs in sandy soil.
Improvement of sludge quality by iron-reducing bacteria
(2004) Ivanov, Volodymyr M.; Wang, J.-Y.; Stabnikov, Victor; Xing, Zikun; Tay, Joo
Sewage sludge can be used in agriculture as organic fertilizer. However, one of the obstacles for this use is the high concentration of heavy metals and the presence of sulphides (acidifying soil or compost). The aim of this research was to develop the biotechnology for improving the quality of sewage sludge that would be used as organic fertilizer. Microbial reduction of inexpensive sources of Fe(III) in anaerobic digester is proposed as a means of preventing the accumulation of sulphide and of enhancing the accumulation of phosphate in sewage sludge. Industrial grade iron hydroxide can serve as a suitable source of Fe(III) The results show that almost all dissolved phosphate is recovered by the reaction with Fe2+. Additionally, the activity of iron-reducing bacteria inhibits the production of sulphide by sulphate-reducing bacteria and the growth of these bacteria in anaerobic digesters.
Influence of starch products on the vitality and activity of lactic acid bacteria in yogurt
(2024) Ivashchenko, Olga; Khonkiv, Myroslav; Stabnikov, Victor; Polishchuk, Galyna; Marynin, Andriy; Buniowska-Olejnik, Magdalena
The influence of starch product with different dextrose equivalents addition on the viability and activity of lactic acid bacteria Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus during fermentation and storage of yogurt has been studied. An increase of dextrose equivalent and monosaccharides content in starch products reduce the fermentation time of milk due to the increase of lactic acid bacteria activity. A slight decrease in water activity in the presence of glucose-fructose syrup in yogurt in an amount of 9% had virtually no effect on the milk fermentation process. The number of lactic acid bacteria increased during the first seven days of yogurt storage added with glucose-fructose syrup. On the 14th day of storage, the concentration of cells of S. thermophilus and L. delbrueckii ssp. bulgaricus became almost the same in all yogurts due to almost complete consumption of carbon sources. When the storage of yogurt was extended to 28 days, the most stable content of lactic acid bacteria was found in yogurt added with maltodextrin due to its prebiotic properties. The increases of active acidity and syneresis in all yogurts were greatest in the first 8–14 days. Presence of dextrins in yogurt stabilizes its physical and chemical properties during storage
Iron-and calcium-based biogrouts for porous soils
(2012) Ivanov, Volodymyr M.; Chu, Jian; Stabnikov, Victor
Chemical grouts are often used to reduce the hydraulic conductivity of soil for seepage control purposes. However, chemical grouts can be expensive and environmentally unfriendly. Therefore, two new biogrouts or microbial-based grouting materials were developed.
Iron-mediated removal of ammonia from strong nitrogenous wastewater of food processing
(2004) Ivanov, Volodymyr M.; Wang, J.-Y.; Stabnikova, Elena; Krasinko, Victoria; Stabnikov, Victor; Tay, Stephen; Tay, Joo
The combination of microbial reduction and further microbial oxidation of iron was applied to the treatment of food-processing wastewater and recovery of ammonium. Fe2+ ions were formed by iron-reducing bacteria under anaerobic conditions. Ammonium was recovered by co-precipitation with negatively charged iron hydroxides produced during oxidation of Fe2+ by iron-oxidizing bacteria under microaerophilic conditions. The value-added by-product of this process can be used as a slowly released ammonium fertilizer.
Microbial method for construction of aquaculture pond in sand
(2013) Chu, Jian; Ivanov, Volodymyr M.; Stabnikov, Victor; Li, Bing
A method to construct an aquaculture pond in sand using microbial biocementation is presented in this paper. The microbially induced calcium carbonate precipitation process was used to form a low-permeability layer in sand for the construction of a water pond model in the laboratory. The test results indicated that the permeability of sand was reduced from the order of 10−4 m/s to 10−7 m/s when an average 2.1 kg of calcium (Ca) per m2 of sand surface was precipitated. The bending strengths of the walls and the base of the model pond were in the range of 90–256 kPa. The unconfined compressive strengths for the samples taken from the walls and the base of the pond were in the range of 215–932 kPa.
Microbially induced calcium carbonate precipitation on surface or in the bulk of soil
(2012) Chu, Jian; Stabnikov, Victor; Ivanov, Volodymyr M.
Microbial precipitation of calcium carbonate takes place in nature by different mechanisms. One of them is microbially induced carbonate precipitation (MICP), which is performed due to bacterial hydrolysis of urea in soil in the presence of calcium ions. The MICP process can be adopted to reduce the permeability and/or increase the shear strength of soil. In this paper, a study on the use of Bacillus sp., which was isolated from tropical beach sand, to perform MICP either on the surface or in the bulk of sand is presented. If the level of calcium salt solution was below the sand surface, MICP took place in the bulk of sand. On the other hand, if the level of calcium salt solution was above the sand surface, MICP was performed on the sand surface and formed a thin layer of crust of calcium carbonate. After six sequential batch treatments with suspension of urease-producing bacteria and solutions of urea and calcium salt, the permeability of sand was reduced to 14 mm/day (or 1.6×10−7 m/s) in both cases of bulk and surface MICP. Quantitiesof precipitated calcium after six treatments were 0.15 and 0.60 g of Ca per cm2 of treated sand surface for the cases of bulk or surface MICP, respectively. The stiffness of the MICP treated sand also increased considerably. The modulus of rupture of the thin layer of crust was 35.9 MPa which is comparable with limestone.
Phosphate removal from return liquor of municipal wastewater treatment plant using iron-reducing bacteria
(2005) Ivanov, Volodymyr M.; Stabnikov, Victor; Zhuang, W. Q.; Tay, Joo; Tay, Stephen
The application of iron-reducing bacteria (IRB) for phosphate removal from return liquor (liquid fraction after activated sludge digestion and anaerobic sludge dewatering) of municipal wastewater treatment plant was studied. Methods and Results: Enrichment culture and two pure cultures of IRB, Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK identified by 16S rRNA gene sequencing, were produced using return liquor of WWTP as carbon and energy source and iron hydroxide as oxidant. The final concentration of phosphate increased from 70 to 90 mg l-1 in control and decreased from 70 to 1 mg l-1 in experiment. The mass ratio of removed P to produced Fe(II) was 0.17 g P g-1 Fe(II). S. maltophilia BK showed the ability to reduce Fe(III) using such xenobiotics as diphenylamine, m-cresol, 2,4-dichlorphenol and p-phenylphenol as sole source of carbon under anaerobic conditions. Bacterial reduction of ferric hydroxide enhanced phosphate removal from return liquor. Significance and Impact of Study: An ability of facultative anaerobes Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK to reduce Fe(III) was shown. These bacteria can be used for anaerobic removal of phosphate and xenobiotics by bacterial reduction of ferric ions.
Screening and selection of microorganisms for the environmental biotechnology process
(2013) Ivanov, Volodymyr M.; Stabnikov, Victor; Hung, Yung-Tse
CONTENT Major physiological groups of microorganisms Periodic table of prokaryotes Use of periodic table for theoretical selection of prokaryotes in environmental engineering Connection between cell shape and physical properties of medium rRNA-based phylogenetic classification cannot be used for theoretical selection in environmental engineering Methods of selection and of isolation of microorganisms Selection of microbial aggregates Growth-related and survival-related selection of microorganisms ///ЗМІСТ Головні фізіологічні групи мікроорганізмів Періодична таблиця прокаріот Використання періодичної таблиці для теоретичної селекції прокаріотів в інженерії довкілля Взаємозв'язок між формою клітини і фізичними властивостями середовища Філогенетична класифікація, що базована на рРНК, не може бути використана для теоретичної селекції в інженерії довкілля Методи селекції та виділення мікроорганізмів Селекція мікробних агрегатів Селекція мікроорганізмів по відношенню до росту та виживання ///СОДЕРЖАНИЕ Главные физиологические группы микроорганизмов Периодическая таблица прокариот Использование периодической таблицы для теоретической селекции прокариотов в инженерии окружающей среды Взаимосвязь между формой клетки и физическими свойствами среды Филогенетическая классификация, основанная на рРНК, не может быть использована для теоретической селекции в инженерии окружающей среды Методы селекции и выделения микроорганизмов Селекция микробных агрегатов Селекция микроорганизмов по отношению к росту и выживаемости
The effect of various iron hydroxide concentrations on the anaerobic fermentation of sulfate-containing model wastewater
(2006) Stabnikov, Victor; Ivanov, Volodymyr M.
Addition of ferric hydroxide and iron-reducing bacteria in anaerobic treatment of sulphate-containing wastewater reduced sulphate reduction and production of sulphide, increased removal of total organic carbon (TOC) and methane production. Influence of ferric addition on sulphate-containing wastewater treatment depended on Fe(III) dosage, which can be determined as a molar ratio of Fe(ІІІ)/SO42-. Concentration of sulphide constantly increased and consisted on 15 day 91 mg/l and 45 mg/l at ratio of Fe(ІІІ)/SO42- 0.06 and 0.5. However, no production of dissolved sulphide was observed at ratio Fe(ІІІ)/SO42- 1 and 2. Maximum rates of total organic carbon removal were 0.75, 1.15, 1.39, 1.55 g TOC/g of volatile suspended solids (VVS) per hour; rates of methane production were 0.039, 0.047, 0.064 and 0.069 ml/g VVS per hour; contents of methane in biogas were 25, 41, 55 and 62 vol. % at the ratios of Fe(ІІІ)/SO42- 0.06, 0.5, 1 and 2, respectively. These data could be used for the development of a new technology for anaerobic treatment of sulphate containing wastewater.
The removal of nitrogen and phosphorus from reject water of municipal wastewater treatment plant using ferric and nitrate bioreductions
(2010) Guo, Cheng Hong; Stabnikov, Victor; Ivanov, Volodymyr M.
Reject water, which is the liquid fraction produced after dewatering of anaerobically digested activated sludge on the municipal wastewater treatment plants (MWWTPs), contributes up to 80% of the nitrogen and phosphorus loads to the MWWTP. It was proposed to combine the removal of nitrogen from reject water using the sequential biooxidation of and bioreduction of NO3- with precipitation of phosphate by Fe2+ ions produced due to bioreduction of Fe3+ in iron ore. Bioreduction of NO3- decreased Fe3+ bioreduction rate in reject water from 37 to 21 mg Fe2+/L d due to competition between NO3- and Fe3+ for electron donors. Addition of acetate as electron donor increased both bioreduction rates of Fe3+ and NO3- but acetate interfered with the competition between nitrate and phosphate anions reacting with ferrous cations decreasing efficiency of the phosphate removal from reject water. The stages of denitrification and ferric bioreduction/phosphate precipitation must be performed sequentially.
The removal of phosphate from wastewater using anoxic reduction of iron ore in the rotating reactor
(2009) Guo, Cheng Hong; Stabnikov, Victor; Kuang, Shengli; Ivanov, Volodymyr M.
The removal of phosphorus from reject water, which is the liquid fraction produced after dewatering of anaerobically digested activated sludge on the municipal wastewater treatment plants (MWWTPs), can significantly reduce the phosphorus load to the main stream of the MWWTPs. Ferric or ferrous reagents can be used for this removal but the significantly cheaper option could be the production of ferrous reagent using bioreduction of iron ore. The removal of phosphorus from reject water using anoxic bioreduction of iron ore was studied in the rotating reactor, which was selected to avoid the clogging of the pores between iron ore particles. The highest phosphorus removal rate from reject water in the rotating reactor, i.e. the parameter which can be used in the design of the continuous process, was 25mg P/L day. Significant role in the iron ore bioreduction is playing the formation of the fine particles from initially loaded coarse particles of iron ore during rotation of the reactor.