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Документ Antimicrobial activity of exocellular metabolites Acinetobacter calcoaceticus IMV V-7241, Rhodococcus erythropolis IMV Ac-5017, Nocardia vaccinii K-8 on Phytopathogenic bacteria(2012) Beregova (Pokora), Khrystyna; Chebotaryova, KseniaWe investigated the effect of exocellular metabolites, including surface- active substances (surfactants) of Rhodococcus erythropolis IMV Ac-5017, Acinetobacter calcoaceticus IMVВ-7241 and Nocardia vaccinii K-8 on somephytopathogenic bacteria. It was shown that after 2 h treatment with surfactant preparations (0.15 - 0.4 mg/mL of strains IMV Ac-5017 and IMV В-7241 survival of genus pathogenic bacteria (105 - 107 in mL of Pseudomonas and Xanthomonas was 0 - 33%. In the presence of a surfactant preparations (0.085 - 0.85 mg/mL), and other exocellular metabolites of N. vaccinii K-8 number of cells of studied phytopathogenic bacteria reduced by 95 - 100 %. The results show the possibility of use microbial surfactants for the development of environmentally friendly products to control the number of pathogenic bacteria.Документ Influence of medium ph on surfactant synthesis by Acinetobacter calcoaceticus IMV B-7241 on hydrophilic substrate ethanol(2012) Antoniuk, Svitlana; Konon, Anastasia; Chebotaryova, KseniaThe synthesis of exocellular metabolites with surface-active and emulsifying properties was investigated at the maintaining pH at 5.0-8.0 rate during the cultivation of Acinetobacter calcoaceticus IMV B-7241 on the medium with ethanol (2% by volume). It was stated that the maintaining pH at 7.0 by the KOH solution was accompanied by the increase of surfactant synthesis in 1.8 times comparing with the process without pH regulation. The replacement of KOH on NaOH for the maintaining pH at an optimal level led to the decrease of surfactant concentration in 1.2-1.5 times that was caused by the inhibitory effect of sodium cations on the activity of enzymes of surface-active amino- and glycolipids biosynthesis.Документ Intensification of surfactant synthesis of Acinetobacter calcoaceticus IMV B-7241 on ethanol in presence of organic acids(2012) Konon, Anastasia; Chebotaryova, KseniaIt was determined that the neutralization of the medium by the KOH solution in the process of Acinetobacter calcoaceticus IMV B-7241 cultivation with the subsequent adding of the fumarate (0.01 %, precursors of the gluconeogenesis) and citrate (0.01 %, the regulator of lipids synthesizes) at the end of the exponential growth phase was accompanied by the increasing surfactant synthesis by 1.2-fold comparing to the same indexes without neutralization and by 3.5-fold comparing to the cultivation of bacteria on ethanol without organic acids and pH regulation. The increasing of the surfactant synthesis in the presence of organic acids was caused by the 1.7-7-fold increased activity of the biosynthesis enzymes of glycolipids and aminolipids as well as the simultaneous functioning of two anaplerotic ways. Було встановлено, що нейтралізація середовища розчином КОН в процесі Acinetobacter calcoaceticus IMV B-7241 культивування з подальшим додаванням фумарату (0,01%, попередники глюконеогенезу) і цитрату (0,01%, регулятор ліпідів синтезує) наприкінці експоненційної фазі росту супроводжувалося збільшенням поверхнево синтезу на 1,2 рази в порівнянні з тими ж індексами без нейтралізації і в 3,5 рази в порівнянні з вирощуванням бактерій на етанолі, без органічних кислот і регулювання рН.