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Документ Impact of germination conditions on antioxidant properties and protein content in lentils (Lens culinaris) of Ukrainian cultivars(2018) Yasinska, Iryna; Ivanova, VictoriaChanges in the free radical scavenging activity, the yield of phenolic compounds, ascorbic acid and proteins content in lentil seeds of two Ukrainian cultivars were determined during the germination period under different light conditions. Our results demonstrate that germinated lentil seeds both cultivars have advantages over non-germinated and can be useful in functional food technologies.Документ Antioxidant activity of extracts from plants growing in Ukraine(2013) Ivanova, Victoria; Yasinska, IrynaRaw materials of some plants growing in Ukraine – the roots of Arctium lappa L., Glycyrrhíza glabra L., Sanguisorba officinalis L., the fruits of Rosa cinnamomea L.- were been used to investigation of its antioxidant properties. The total phenolic content of prepared aqueous extracts was determined using Folin-Ciocalteu reagent; it ranged from 43.2±2.09 to 98.5±4.01 mg GAE per 100 ml of the extract. The highest vitamin C content was found in the extracts from the rose hips and the great burnet roots - 8.94±0.234 and 7,68±0.341 mg / 100 ml of extract, respectively. Free radical scavenging capacity of the extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH). It ranged from 0.60± 0.0178 to 8.45± 0.26 mM of the ascorbic acid equivalent (AAE) antioxidant capacity per 100 ml of the extract. The highest antioxidant activity was determined for the extract of great burnet roots. The results showed that tested extracts possess potent antioxidant activity and may be considered as natural source of antioxidants.Документ Constitutive expression of recombinant clumping factor of Staphylococcus aureus in Escherichia coli(2003) Ivanova, Victoria; Pozur, V.Experiments for optimization of the clfA gene expression in E.coli BL21 (DE3)Gold cells were conducted. It has been shown the reducing of the strain efficiency and the elimination of the plasmid during the cultivation of a producer. Several methods for raising the amount of plasmid-containing cells and increasing the yield of recombinant protein were proposed. Bacterial growth medium was selected to make this process more efficient. In such conditions after IPTG induction of the recombinant cells we have got high level of a soluble protein (about 30 % of total cellular protein).