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Постійне посилання колекціїhttps://dspace.nuft.edu.ua/handle/123456789/7522

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  • Ескіз
    Документ
    Use of cells and surfactants of Nocardia vaccinii K-8 in bioremediation processes
    (2012) Gritsenko (Manzhula), Natalia; Sofіlkanich (Morozova), Anna; Konon, Anastasia
    The possibility of use of Nocardia vaccinii K-8 cells as well as their metabolites for remediation of oil polluted ecosystems was studdied. It was shown that the highest oil destruction degree (94-98 %) in polluted water (2.6 g/L) was achieved in the case of treatment with suspension of N. vaccinii K-8 cells (9.8107 CFU/mL) after 30 days, while surfactant preparation of post fermentative cultural liquid (100300 mL/kg) was more effective for remediation (destruction of 74-83 % of oil) of oil polluted soil (20 g/kg). Furthermore, the introduction of 30 ml of these preparation to the oil polluted sand (0.1 mL of oil/1 g of sand) resulted in detachment of 90 % of oil.
  • Ескіз
    Документ
    Biosurfactants of Rhodococcus erythropolis IMV Ас-5017: synthesis intensification and practical application
    (2013) Pirog, Tatiana; Sofіlkanich (Morozova), Anna; Shulyakova, Mariya; Shevchuk, Tetiana
    Intensification of the surfactant synthesis by Rhodococcus erythropolis IMV Ac-5017 on different substrates, including industrial waste, as well as the use of surfactant preparations for oil degradation were studied. It was established that the addition of fumarate (0.2 %) and citrate (0.1 %) into the medium with ethanol, n-hexadecane, or glycerol (1–2 %) was accompanied by an increase of conditional surfactant concentration by 1.5–1.7 times compared to the indexes in the medium without organic acids. The intensification of surfactant synthesis in the presence of fumarate and citrate is caused by the increased activity of isocitrate lyase (by 1.2–15-fold) and enzymes of the surfactant biosynthesis (by 2–4.8-fold) compared to their activity in the medium without precursors. The possibility of surfactant synthesis intensification (by 3–4-fold) while cultivating of R. erythropolis IMV Ac-5017 in the medium with oil containing substrates (2 %) and glucose (0.1 %) was shown. The introduction of 0.01 mM Cu2+ in the exponential growth phase of strain IMV Ac-5017 in the medium with ethanol accompanied by the increasing conditional surfactant concentration by 1.9 times. The highly efficient remediation (92–95 %) of oil (2–2.6 g/L) and Cu2+ polluted water after treatment with surfactant preparations (native cultural liquid) at low concentrations (5 %) was determined.