The evaluation of cell integrity by immediate hexane extraction (shaking method) showed that all samples treated with different enzymes had a higher oil yield, ranging from 33.2 to 34.1 % of seed weight, than the control samples (32.1 %). The number of disrupted cells in enzyme treated samples was also higher than the control (64.4%), ranging from 67.6 to 69.5 %. The highest amount of damaged cells, 71.0 and 71.1 %, was found in the samples treated with mixtures (a) pepsin, Viscozyme L, pectinase and (b) pepsin, cellulase,pectinase. The yield of pressed oil from pumpkin seeds pretreated with the pepsin+ViscozymeL+pectinase mixture was by 7.0 % higher than that of the control sample. The enzymatic pretreatment did not significantly affect the free fatty acid content, peroxide value, fatty acid, and phytosterols composition. The antioxidant activity expressed as DPPH radical scavenging capacity of the pressed oil obtained from enzymatically pretreated pumpkin seeds was by 2.7 % higher than of control oil.
