Effective expression of recombinant Staphylococcus aureus clumping factor and it immunobiological activity
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Abstract
Fragments of the clfA gene coding for clumping factor of S.aureus were cloned into the expression plasmid pQE30 in E.coli XLI-Blue cells. It has been shown the reducing of the strain efficiency and the elimination of the plasmid during the cultivation of a producer. Several methods for raising the amount of plasmid-containing cells and increasing the yield of recombinant protein were proposed.
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clumping factor, пластівцевоутврюючий фактор, хлопьеобразующий фактор, Staphylococcus aureus, IPTG, stability of the plasmid, cultivation, expression, золотистий стафілокок, стабільність плазміди, культивування, експресія, золотистый стафилококк, стабильность плазмиды, культивирования, экспрессия, кафедра експертизи харчових продуктів
Citation
Ivanova, V. Effective expression of recombinant Staphylococcus aureus clumping factor in E.coli cells and it immunobiological activity / V. Ivanova // Conference for students, PhD students and young scientists on molecular biology and genetics. Dedicated to the establishment of Ukrainian Society for molecular biology. – Kyiv (Ukraine). – 2001. – P. 96.