Тези доповідей, матеріали конференцій
Постійне посилання на розділhttps://dspace.nuft.edu.ua/handle/123456789/7373
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Документ Biosynthesis of silver nanoparticles using Acinetobacter calcoaceticus ІМV V-7241 cell-free supernatant(2020) Laziuka, Yuliya; Kharchenko, Yevgen; Skrotska, OksanaCurrently, the biological synthesis of nanoparticles and their properties and application in the fields of medicine and the environment is an important area of nanotechnology. Synthesis of nanoparticles using chemical and physical methods has several disadvantages. These drawbacks include the use of toxic solvents, the formation of dangerous by-products and high energy consumption. Therefore, it is important to obtain nanoparticles in a biogenic way, in particular by microorganisms. The mechanisms of biogenic synthesis of nanoparticles have not been fully studied. There are reports on the role of surface active agents (surfactants) in this process. Therefore, the producer of surfactants Acinetobacter calcoaceticus ІМВ В-7241 (which was isolated from the oil contaminated environment) was selected for the study.Документ Biosynthesis of recombinant interferons in transgenic plants(2017) Holubiev, Pavlo; Kharchenko, Yevgen; Skrotska, OksanaThe production of recombinant cytokines with transgenic plants may solve the problem of high demand on drugs against severe diseases, such as hepatitis, AIDS, chronic granulomatous disease, etc. Microorganisms, mammal cell cultures and even plants may be involved in interferon (IFN) production. Considering an array of disadvantages of microbial and mammalian cell cultures, transgenic plants become an object of interest as a perspective producer of interferon.Документ Ecological synthesis of silver nanoparticles using bacteria(2020) Laziuka, Yuliya; Skrotska, Oksana; Kharchenko, YevgenDue to the potential use of nanomaterials, there is a need to investigate environmentally friendly technologies for the synthesis of nanoparticles. Traditionally, nanoparticles are synthesized by physical and chemical methods. However, these methods are characterized by the use of hazardous and aggressive substances, which are harmful to the environment and cause serious health problems. It should be noted that the production of nanoparticles using microorganisms, in particular bacteria, is environmentally safe, since this method of synthesis does not require the use of toxic materials.Документ Saccharomycetes as biological agents for the mediated biosynthesis of silver nanoparticles(2021) Kharchenko, Yevgen; Skrotska, OksanaThe most commonly used are chemical and physical methods of silver nanoparticles (AgNPs) synthesis, but there is also biological synthesis, which is a promising and more environmentally friendly method. Synthesized using the supernatant Saccharomycescerevisiae M437 AgNPs had a spherical shape. AgNPs that were obtained using a cell-free aqueous extract of S. cerevisiae M437 had an oval shape.Документ Biosynthesis of silver nanoparticles by Saccharomyces cerevisiae M437(2021) Kharchenko, Yevgen; Skrotska, Oksana; Laziuka, Yuliya; Marchenko (Potapenko), ValeriaSilver nanoparticles (AgNPs) are widely used in medicine and pharmaceuticals as biosensors, diagnostic agents, targeted drug delivery agents with controlled release, as antimicrobial, and anticancer agents. AgNPs are mainly produced by chemical synthesis. The disadvantages of this method: the use of toxic substances (ethylene glycol, hydrazine, and sodium borohydride), low yield of nanoparticles, expensive isolation, and purification. Therefore, it is important to find a safe and effective synthesis method, for example using green synthesis. Biosynthesis can provide a high yield of particles stabilized by natural substances that have high biocompatibility. Biological synthesis can take place both intracellular and extracellular. Cell extracts can also be used for biosynthesis. In this study, the purpose was to synthesize silver nanoparticles using metabolites contained in the supernatant of Saccharomyces cerevisiae M437.