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Документ Modernization of the Pseudomonas syringae pathovars serogrouping scheme(2022) Pasichnyk, Lidiia; Butsenko, LiudmylaIn 1979, L.T. Pastushenko and I.D. Symonovych developed a scheme of serogrouping phytopathogenic bacteria of the Pseudomonas genus, which is still used now. However, today's using this serogrouping scheme is complicated by the lack of all data accumulated over the years of its application. Moreover, the scheme does not correspond to the modern taxonomy of phytopathogenic bacteria of the Pseudomonas genus. Aim. On the basis of own experimental results and data of scientific literature, to carry out modernization of the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas genus. Methods. The strains of Pseudomonas syringae pathovars such as atrofaciens, coronafaciens, tabaci, which were isolated from plants of wheat, rye, oats, tobacco, and various species of affected weeds in different regions of Ukraine have been studied in the work. Antigenic properties of bacterial strains were studied by agglutination and precipitation reactions (the Ouchterlony double immunodiffusion techniques) using antisera to P. syringae strains of nine serological groups (I, II, III, IV, V, VI, VII, VIII, IX). To carry out the precipitation reaction, O- and OH-antigens were obtained by a modified Grasse's method. The presence of the same number of precipitation lines of the studied antigens as the number of lines with homologous antiserum of the corresponding serogroup testified to their belonging to this serogroup according to the known serogrouping scheme of phytopathogenic bacteria developed in 1979 by L.T. Pastushenko and I.D. Symonovych. Results. It has been proved that strains of P. syringae pathovars isolated from different cereals (rye, wheat, oats) and segetal vegetation differ in antigenic composition. The antigenic composition of P. syringae strains depends on the host plant from which the pathogen was isolated. Strains of the causative agent of basal glume rot P. syringae pv. atrofaciens isolated from wheat belong to four serological groups (II, IV, V, VI), from rye - to five serological groups (I, II, IV, V, VI), as well as strains of this pathogen isolated from segetal vegetation of wheat agrophytocenosis. Strains of the halo bligth P. syringae pv. coronafaciens from affected oat plants belong to two serological groups (I, V). The serogrouping scheme has been supplemented by new data on the antigenic properties of P. syringae pv. tomato, the causative agent of the bacterial speck disease of tomatoes (Solanum lycopersicum), which is classified as serogroup IV. It has been found that P. syringae pv. tabaci strains, which cause wildfire of tobacco, are part of three serogroups - VII, VIII, IX, and not of two ones, as was presented in the known scheme (1979). Conclusions. Therefore, on the basis of our own research and literature data, the serogrouping scheme of phytopathogenic bacteria of the Pseudomonas genus developed by L.T. Pastushenko and I.D. Symonovych has been modernized. In the renovated scheme, current species' names of phytopathogens are present, this scheme introduces new information about the serogroups of P. syringae pv. atrofaciens isolated from rye seeds and plants, weeds, P. syringae pv. coronafaciens - from oat plants, P. syringae pv. tabaci - from tobacco, and P. syringae pv. tomato - from affected tomato plants.Документ Methodology for determining the physical parameters of wastewater(2020) Isaenko, Volodymyr; Frolov, Valery; Madzhd, Svitlana; Mashkov, OlehMethodology for determining the physical parameters of wastewater discharge into surface water bodies of different types has been developed, which allows to determine the precipitation kinetics of wastewater impurities and their dependence on concomitant factors. The proposed methodology allows, based on the hydraulic size, to determine the deposition rate of particles of different size and shape contained in the wastewater and to determine the factors that affect the process of different shapes and masses particles mixing. The algorithm of measures of preliminary sewage treatment by enterprises before discharge into surface reservoirs is offered in the work. The implementation of the proposed algorithm will determine the impact of wastewater on the quality and chemically-biological composition of surface water bodies of Ukraine, as well as significantly improve the quality characteristics of surface and groundwater, which are water supply source for all residents of cities and towns of UkraineДокумент Вплив кліматичних умов на основні показники озимого ячменю сорту Казанова(2019) Кошова, Валентина Миколаївна; Коберніцька, Аліна Олександрівна; Смажко, Ірина ОлександрівнаДана науково-дослідна робота направлена на вдосконалення технології пивоварного солоду з озимого дворядного ячменю сорту Казанова українського виробництва, дослідження біохімічних перетворень у зерні під впливом кліматичних умов. Як дослідний зразок було обрано сорт озимого ячменю Казанова 2017, 2018 і 2019 року врожаю, в якому визначались фізико-хімічні та фізіологічні показники.