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  • Ескіз
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    Вплив додавання заліза на процес виділення фосфату при анаеробному зброджуванні активного мулу
    (2004) Стабніков, Віктор Петрович; Решетняк, Людмила Расуловна
    Проведено дослідження впливу додавання нерозчинених сполук трьохвалентного заліза на процес виділення фосфату при анаеробом зброджуванні активного мулу. Показано, що для ефективного видалення фосфату співвідношення кількості відновленого заліза до фосфату повинна становити 1,4 – 1,6. Ефективність видалення фосфату при високій дозі внесеного заліза складала 95% від початкового значення. При концентрації Fe(III) 75 -150 мг/л все внесене залізо було відновлено за 10 діб анаеробного процесу. При підвищенні вмісту внесеного Fe(III) знижувалася кількість біогазі, але підвищувалося співвідношення СН4:СО2. Дані можуть бути застосовано підстави для розробки нової технології анаеробного зброджування активного мулу. Addition of ferric hydroxide to the reactor of anaerobic digestion of activated sludge followed microbial reduction of Fe (III) and formation of ferrous ions, which precipitated phosphate. It was shown that the ratio of reduced iron to removed phosphate must be 1.4 – 1.6 to achieve efficient phosphate removal from the liquid of anaerobic digester. Efficiency of phosphate removal in the experiment with high dosage of iron was 95%. If concentration of Fe(III) was 75 -150 mg/l, all added ferric was reduced for 10 days of anaerobic process. The volume of biogas decreased from 343 mg/l to 293 mg/l, but the ratio of СН4 : СО2 increased from 2,2 tо 3,1, when the concentration of Fe(III) changed from 75 mg/l tо 600 mg/l. The results could be used to develop new technology of anaerobic digestion of activated sludge.
  • Ескіз
    Документ
    Improvement of sludge quality by iron-reducing bacteria
    (2004) Ivanov, Volodymyr M.; Wang, J.-Y.; Stabnikov, Victor; Xing, Zikun; Tay, Joo
    Sewage sludge can be used in agriculture as organic fertilizer. However, one of the obstacles for this use is the high concentration of heavy metals and the presence of sulphides (acidifying soil or compost). The aim of this research was to develop the biotechnology for improving the quality of sewage sludge that would be used as organic fertilizer. Microbial reduction of inexpensive sources of Fe(III) in anaerobic digester is proposed as a means of preventing the accumulation of sulphide and of enhancing the accumulation of phosphate in sewage sludge. Industrial grade iron hydroxide can serve as a suitable source of Fe(III) The results show that almost all dissolved phosphate is recovered by the reaction with Fe2+. Additionally, the activity of iron-reducing bacteria inhibits the production of sulphide by sulphate-reducing bacteria and the growth of these bacteria in anaerobic digesters.
  • Ескіз
    Документ
    Видалення фосфату з рідинної фракції анаеробного реактору і застосування його як добрива
    (2005) Стабніков, Віктор Петрович; Решетняк, Людмила Расуловна
    Показано можливість видалення фосфату з рідинної фракції анаеробного реактора міських водоочисних споруд за допомогою накопичувальної культури залізовідновлювальних бактерій при використанні гідроксиду заліза як джерела Fe(III). Наведено результати дослідження застосування отриманого фосфат-залізовмісного осаду як фосфорного добрива. The possibility of phosphate removal from liquid fraction from anaerobic reactor of municipal wastewater treatment plant by enrichment culture of iron-reducing bacteria with the ferric hydroxide addition was shown. The results of produced phosphate - iron precipitate application as phosphorous fertilizer were presented.
  • Ескіз
    Документ
    Application of iron-reducing bacteria for phosphate removal from returned liquor of municipal wastewater treatment plant
    (2005) Ivanov, Volodymyr M.; Stabnikov, Victor; Tay, Stephen; Tay, Joo
    The aim of this research was to examine efficiency of enrichment culture and isolated strains of iron-reducing bacteria for the removal of phosphate from return liquor of municipal wastewater treatment plant (MWWTP) with ferric hydroxide as a source of Fe (III). Bacterial reduction of ferric hydroxide enhanced phosphate removal from return liquor. The obtained data could be used for the design of a new biotechnology of anaerobic removal or recovery of phosphate from return liquor of MWWTP.
  • Ескіз
    Документ
    Phosphate removal from return liquor of municipal wastewater treatment plant using iron-reducing bacteria
    (2005) Ivanov, Volodymyr M.; Stabnikov, Victor; Zhuang, W. Q.; Tay, Joo; Tay, Stephen
    The application of iron-reducing bacteria (IRB) for phosphate removal from return liquor (liquid fraction after activated sludge digestion and anaerobic sludge dewatering) of municipal wastewater treatment plant was studied. Methods and Results: Enrichment culture and two pure cultures of IRB, Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK identified by 16S rRNA gene sequencing, were produced using return liquor of WWTP as carbon and energy source and iron hydroxide as oxidant. The final concentration of phosphate increased from 70 to 90 mg l-1 in control and decreased from 70 to 1 mg l-1 in experiment. The mass ratio of removed P to produced Fe(II) was 0.17 g P g-1 Fe(II). S. maltophilia BK showed the ability to reduce Fe(III) using such xenobiotics as diphenylamine, m-cresol, 2,4-dichlorphenol and p-phenylphenol as sole source of carbon under anaerobic conditions. Bacterial reduction of ferric hydroxide enhanced phosphate removal from return liquor. Significance and Impact of Study: An ability of facultative anaerobes Stenotrophomonas maltophilia BK and Brachymonas denitrificans MK to reduce Fe(III) was shown. These bacteria can be used for anaerobic removal of phosphate and xenobiotics by bacterial reduction of ferric ions.
  • Ескіз
    Документ
    The effect of various iron hydroxide concentrations on the anaerobic fermentation of sulfate-containing model wastewater
    (2006) Stabnikov, Victor; Ivanov, Volodymyr M.
    Addition of ferric hydroxide and iron-reducing bacteria in anaerobic treatment of sulphate-containing wastewater reduced sulphate reduction and production of sulphide, increased removal of total organic carbon (TOC) and methane production. Influence of ferric addition on sulphate-containing wastewater treatment depended on Fe(III) dosage, which can be determined as a molar ratio of Fe(ІІІ)/SO42-. Concentration of sulphide constantly increased and consisted on 15 day 91 mg/l and 45 mg/l at ratio of Fe(ІІІ)/SO42- 0.06 and 0.5. However, no production of dissolved sulphide was observed at ratio Fe(ІІІ)/SO42- 1 and 2. Maximum rates of total organic carbon removal were 0.75, 1.15, 1.39, 1.55 g TOC/g of volatile suspended solids (VVS) per hour; rates of methane production were 0.039, 0.047, 0.064 and 0.069 ml/g VVS per hour; contents of methane in biogas were 25, 41, 55 and 62 vol. % at the ratios of Fe(ІІІ)/SO42- 0.06, 0.5, 1 and 2, respectively. These data could be used for the development of a new technology for anaerobic treatment of sulphate containing wastewater.
  • Ескіз
    Документ
    The removal of phosphorus from reject water of municipal wastewater treatment plant using iron ore
    (2009) Guo, Cheng Hong; Stabnikov, Victor; Kuang, Shengli; Ivanov, Volodymyr M.
    BACKGROUND: Reject water (return liquor) from dewatering of anaerobically digested activated sludge in municipal wastewater treatment plants contains from 10 to50%of the phosphorus load when being recycled to the aeration tank. Phosphorus removal from reject water could be an effective way to decrease phosphorus loads entering the aeration tank. An innovative approach involves the replacement of iron salts, which are commonly used for phosphorus removal, with ferrous ions produced by iron-reducing bacteria from iron ore. The aim of the research was to examine the feasibility of phosphorus removal from return liquor using bioreduction of iron ore.RESULTS: Ferrous production, phosphate and organic carbon removal rates were determined as a function of different iron ore particle sizes in batch experiments. Iron-reducing bacteria ensured the production of ferrous ions from iron ore up to concentrations of 550 mg L−1. The ferrous production rate was linearly dependent on the calculated specific surface area of the iron ore particles. The phosphorus concentration in the reject water was reduced by 90% during bioreduction of iron ore. The phosphorus removal rate did not depend on specific surface area of iron ore particles when the particle size of iron ore was smaller than 7 mm. The organic carbon removal rate did not seem to be dependent on iron ore particle size. CONCLUSION: Removal of phosphate using iron ore can be more economical than conventional chemical precipitation of phosphate using iron salts because of the lower cost of iron ore.
  • Ескіз
    Документ
    Вплив гідроксиду заліза на анаеробне зброджування сульфатвмісних стічних вод
    (2004) Стабніков, Віктор Петрович; Красінько, Вікторія Олегівна; Решетняк, Людмила Расуловна
    Стічні води рибопереробної промисловості та багатьох біотехнологічних підприємств містять значну кількість сульфатів. Метою даної роботи було вивчення впливу гідроксиду трьохвалентного заліза на процес анаеробного зброджування стічних вод, що містили органічні речовини та сульфат. Показано, що при внесенні гідроксиду трьохвалентного заліза при анаеробному зброджуванні стічних вод з високим вмістом органічних речовин і сульфату інтенсифікується процес очищення завдяки діяльності залізовідновлювальних бактерій. При додаванні гідроксиду заліза в модельну сульфатвмісну стічну воду сірководень був повністю відсутнім в біогазі і рідкій фазі. Співвідношення числа сульфатредукуючих бактерій та метаногенів після додавання гідроксиду заліза знижувалося у 6 разів. Wastewater of fish-processing industry and many biotechnological plants contain the significant amount of sulfates. An aim of the present research was to study the effect of iron hydroxide on the anaerobic fermentation of wastewater containing organic substances and sulfate. It was shown that addition of ferric hydroxide during anaerobic treatment of wastewater with high content of organic substances and sulfate intensified purification process due to the activity of iron-reducing bacteria. Hydrogen sulfide was absent in biogas and in the liquid phase when iron hydroxide was added to the model sulfate-containing wastewater. Ration of sulfate-reducing bacteria and the methanogens diminished in 6 times after iron hydroxide addition.
  • Ескіз
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    Влияние гидроокисида железа на анаэробное сбраживание сульфатсодержащих сточных вод
    (2004) Стабников, Виктор Петрович; Иванов, Владимир Николаевич; Решетняк, Людмила Расуловна; Тэй, С. Т. Л.
    Исследовали влияние гидроксида трехвалентного железа на процесс анаэробного сбраживания сточных вод, содержащих органические вещества и сульфаты. Трехвалентное железо восстанавливалось до Fе(II) железовосстанавливающими бактериями. Это сопровождалось повышением качества очистки, что проявлялось в снижении конечного ХПК на 33,1% и увеличении объема образованного биогаза на 15,9 % по сравнению с процессом без внесения железа, а также отсутствием сероводорода в биогазе и жидкости. Соотношение числа клеток сульфатредуцирующих бактерий и метаногенов уменьшалось в 6 раз после добавления гидроксида железа
  • Ескіз
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    Iron-mediated removal of ammonia from strong nitrogenous wastewater of food processing
    (2004) Ivanov, Volodymyr M.; Wang, J.-Y.; Stabnikova, Elena; Krasinko, Victoria; Stabnikov, Victor; Tay, Stephen; Tay, Joo
    The combination of microbial reduction and further microbial oxidation of iron was applied to the treatment of food-processing wastewater and recovery of ammonium. Fe2+ ions were formed by iron-reducing bacteria under anaerobic conditions. Ammonium was recovered by co-precipitation with negatively charged iron hydroxides produced during oxidation of Fe2+ by iron-oxidizing bacteria under microaerophilic conditions. The value-added by-product of this process can be used as a slowly released ammonium fertilizer.