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Документ Determining the influence of plant-based proteins on the characteristics of dairy ice cream(2024) Mykhalevych, Artur; Polishchuk, Galyna; Bandura (Kuzmyk), Uliana; Osmak (Fedchenko), Tetiana; Bass, OksanaThis paper investigates the functional and technolog-ical properties of proteins in the composition of dairy ice cream. The object of the research was the technology of ice cream with plant-based proteins. The problem to be solved was the improvement of physical-chemical and rheolog-ical characteristics of mixtures and ice cream with a low fat content by using moisture-binding structuring proteins of plant originДокумент Effects of protein and carbohydrate ingredients on colour of baked milk products(2024) Mandiuk, Olena; Lohinova, Anna; Arsenieva, Larisa; Petrusha, Oksana; Polishchuk, GalynaIn the CIE Lab system, only the coordinates “a” and “b” should be used to characterize the color change of milk of 2.5% fat and cream of 10% fat during heat treatment at 95–97 ⁰C for 160–180 min, as the L indicator (light level) is not sufficiently informative. According to the selected coordinates, rational ranges were established as a criterion for the completeness of the Maillard reaction for baked milk and cream, in particular for coordinate “a” in the range from 1.5 to 2.0 units, for coordinate “b” from 11.5 to 13.0 units. The application of whey protein concentrate, hydrolysed demineralized whey concentrate, and glucose-fructose syrup, which contain monosaccharides and proteins, significantly enhanced the Maillard reaction. The recommended values for color coordinates of cream with milk protein and carbohydrate ingredients were achieved during the simmering process. For cream with whey protein concentrate, this occurs at a minimum of 21 min; for cream with hydrolysed whey concentrate at a minimum of 28 min, and for cream with glucose-fructose syrup and whey protein concentrate at a minimum of 18 min. The samples with whey protein concentrate and glucose-fructose syrup, including those one with whey protein concentrate, showed an excellent level of quality in terms of sensory characteristics after 20 min, while the sample with hydrolysed whey concentrate demonstrated this after 30 min of simmering. These results correlated with the rational duration of cream simmering to achieve the recommended degree of color. A slight decrease in acidity was observed in all cream samples during the heating process. The reduction in the duration of the simmering process of dairy products with simultaneous achievement of recommended color characteristics will contribute to a significant reduction in heat energy consumptionДокумент Influence of starch products on the vitality and activity of lactic acid bacteria in yogurt(2024) Ivashchenko, Olga; Khonkiv, Myroslav; Stabnikov, Victor; Polishchuk, Galyna; Marynin, Andriy; Buniowska-Olejnik, MagdalenaThe influence of starch product with different dextrose equivalents addition on the viability and activity of lactic acid bacteria Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus during fermentation and storage of yogurt has been studied. An increase of dextrose equivalent and monosaccharides content in starch products reduce the fermentation time of milk due to the increase of lactic acid bacteria activity. A slight decrease in water activity in the presence of glucose-fructose syrup in yogurt in an amount of 9% had virtually no effect on the milk fermentation process. The number of lactic acid bacteria increased during the first seven days of yogurt storage added with glucose-fructose syrup. On the 14th day of storage, the concentration of cells of S. thermophilus and L. delbrueckii ssp. bulgaricus became almost the same in all yogurts due to almost complete consumption of carbon sources. When the storage of yogurt was extended to 28 days, the most stable content of lactic acid bacteria was found in yogurt added with maltodextrin due to its prebiotic properties. The increases of active acidity and syneresis in all yogurts were greatest in the first 8–14 days. Presence of dextrins in yogurt stabilizes its physical and chemical properties during storageДокумент The influence of whey protein isolate on the quality indicators of acidophilic ice cream based on liquid concentrates of demineralized whey(2024) Mykhalevych, Artur; Buniowska-Olejnik, Magdalena; Polishchuk, Galyna; Puchalski, Czesław; Kaminska-Dwórznicka, Anna; Berthold-Pluta, AnnaThe use of liquid whey concentrates in the composition of ice cream, especially in combination with other powdered whey proteins, is limited due to their understudied properties. This article shows the main rheological and thermophysical characteristics of ice cream mixes, as well as color parameters, microstructure, analysis of ice crystals and quality indicators of ice cream during storage. The most significant freezing of free water (p ≤ 0.05) was observed in the temperature range from the cryoscopic temperature to −10 ◦C. The microscopy of experimental ice cream samples based on hydrolyzed whey concentrates indicates the formation of a homogeneous crystalline structure of ice crystals with an average diameter of 13.75–14.75 µm. Microstructural analysis confirms the expediency of using whey protein isolate in ice cream, which ensures uniform distribution of air bubbles in the product and sufficient overrun (71.98–76.55%). The combination of non-hydrolyzed whey concentrate and 3% whey protein isolate provides the highest stability to preserve the purity and color intensity of the ice cream during storage. The produced ice cream can be classified as probiotic (number of Lactobacillus acidophilus not lower than 6.2 log CFU/g) and protein-enriched (protein supply from 15.02–18.59%).Документ Influence of plant-based structuring ingredients on physicochemical properties of whey ice creams(2024) Tomczynska-Mleko, Marta; Mykhalevych, Artur; Sapiga, Victoria; Polishchuk, Galyna; Terpiłowski, Konrad; Mleko, Stanislaw; Sołowiej, Bartosz; Pérez-Huertas, SalvadorThe dairy industry is actively seeking newapplications for various types ofwhey. One promising direction is the development of nutritious ice cream, using a blend of different whey proteins. However, the production of whey ice cream is hindered by the occurrence of quality issues, primarily stemming froma low content of solids, particularly fat and protein. The development of natural components with distinctive technological attributes, such as the ability to bind excessmoisture, enhance foaming properties, and replicate the taste of milk fat, is of significant relevance in food science. In this work, we investigated the influence of plant-based structuring ingredients on the viscoelastic characteristics of whey-based ice creams. Notably,mixes such as 0.4% Vianoks C45 + 0.75% oat β-glucan, 0.4% Vianoks C45 + 0.5% yeast β-glucan, and 0.4% Vianoks C45 + 3% whey protein complex + 10% vegetable purée from table beet have been proven to be effective stabilizing compositions. However, attempts to combine the whey protein complexwith other types of vegetable purées like zucchini and broccoli did not yield satisfactory results. It has also been found that β-glucan from the yeast Saccharomyces cerevisiae and κ-carrageenan, a component of the Vianoks C45 stabilization system, forms a robust gel within the system. Analysis of the aqueous phase in whey-based ice creams revealed a consistent correlation between water activity, surface tension, and rheological behavior. Finally, the ice creams that exhibited the best viscoelastic characteristics also had the best sensory attributes.Документ Functional and technological properties of protein ingredients in whey ice cream(2022) Mykhalevych, Artur; Polishchuk, Galyna; Buniowska-Olejnik, Magdalena; Tomczynska-Mleko, Marta; Mleko, StanislawThis study explores rheological characteristics of mixes of whey ice cream with protein ingredients. The viscosity characteristics of the mixes were studied by the method of rotational viscometry. Foam overrun and foam resistance of ice cream mixes were determined using modified methods. The expediency of using protein ingredients in whey ice cream mixes to increase their nutritional value was shown. Addition of whey protein isolate in the amount of 3-5%, micellar casein, 3%, or whey protein concentrate, 3%, increased the foam overrun of whey ice cream mixes, while addition of soy protein isolate decreased it. The highest rate of foam resistance, 57.6-58.4%, was recorded for the mix with 3-5% of whey protein isolate. Based on the results of the analysis of flow rheograms of ice cream mixes with various protein ingredients, they were classified as food systems with a coagulation structure, characterized by pronounced thixotropic properties. In the case of micellar casein, whey protein concentrate or whey protein isolate, the thixotropic ability of the mixes increases from 58.2% to 62.2-72.2%. Soy protein isolate does not show the specified technological activity. The highest thixotropy of the mixes was observed for mixes with 3% micellar casein and 3-5% whey protein isolate due to their specific ability to form a spatial coagulation structure, which spontaneously restores the structure after destruction due to the presence of numerous low-energy bonds. Whey protein concentrate has a moderate effect on the rheological characteristics of the mixes, while the presence of soy protein isolate leads to a partial loss of the ability of the mixes to spontaneously restore the destroyed structure. The possibility to increase the total protein content in ice cream from 3.45% to 6.02-7.81% due to the use of technologically effective milk-protein ingredients has been proven. Micellar casein and whey protein isolate in the composition of whey ice cream mixes show high technological activity and significantly improve the quality indicators of the finished product. Це дослідження вивчає реологічні характеристики сумішей сироваткового морозива з білковими інгредієнтами. В'язкісні характеристики сумішей досліджували методом ротаційної віскозиметрії. Піностійкість сумішей для морозива визначали модифікованими методами. Показано доцільність використання білкових інгредієнтів у сумішах сироваткового морозива для підвищення їх харчової цінності. Додавання ізоляту сироваткового протеїну в кількості 3-5%, міцелярного казеїну 3% або концентрату сироваткового білка 3% підвищувало піноутворення сумішей сироваткового морозива, а додавання ізоляту соєвого білка зменшувало його. Найвищий показник піностійкості 57,6-58,4% зафіксований для суміші з 3-5% ізоляту сироваткового протеїну. За результатами аналізу реограм течії сумішей для морозива з різними білковими інгредієнтами їх віднесено до харчових систем з коагуляційною структурою, що характеризуються вираженими тиксотропними властивостями. У випадку міцелярного казеїну, концентрату сироваткового білка або ізоляту сироваткового білка тиксотропна здатність сумішей підвищується з 58,2% до 62,2-72,2%. Ізолят соєвого білка не виявляє зазначеної технологічної активності. Найвищу тиксотропність сумішей спостерігали у сумішей з 3% міцелярного казеїну та 3-5% ізоляту сироваткового білка через їх специфічну здатність утворювати просторову коагуляційну структуру, яка спонтанно відновлює структуру після руйнування за рахунок наявності численних низьких енергетичні зв'язки. Концентрат сироваткового протеїну має помірний вплив на реологічні характеристики сумішей, тоді як наявність ізоляту соєвого білка призводить до часткової втрати здатності сумішей до самовільного відновлення зруйнованої структури. Доведено можливість підвищити вміст загального білка в морозиві з 3,45% до 6,02-7,81% за рахунок використання технологічно ефективних молочно-білкових інгредієнтів. Міцелярний казеїн та ізолят сироваткового протеїну у складі сумішей для сироваткового морозива виявляють високу технологічну активність та значно покращують якісні показники готової продукції.Документ Co-Gelation of Pumpkin-Seed Protein with Egg-White Protein(2023) Tomczynska-Mleko, Marta; Terpiłowski, Konrad; Pérez-Huertas, Salvador; Sapiga, Victoria; Polishchuk, Galyna; Sołowiej, Bartosz; Nastaj, Maciej; Wesołowska-Trojanowska, Marta; Mleko, StanislawThe aim of this study was to investigate the gelation process of binary mixes of pumpkin-seed and egg-white proteins. The substitution of pumpkin-seed proteins with egg-white proteins improved the rheological properties of the obtained gels, i.e., a higher storage modulus, lower tangent delta, and larger ultrasound viscosity and hardness. Gels with a larger egg-white protein content were more elastic and more resistant to breaking structure. A higher concentration of pumpkin-seed protein changed the gel microstructure to a rougher and more particulate one. The microstructure was less homogenous, with a tendency to break at the pumpkin/egg-white protein gel interface. The decrease in the intensity of the amide II band with an increase in the pumpkin-seed protein concentration showed that the secondary structure of this protein evolved more toward a linear amino acid chain compared with the egg-white protein, which could have an impact on the microstructure. The supplementation of pumpkin-seed proteins with egg-white proteins caused a decrease in water activity from 0.985 to 0.928, which had important implications for the microbiological stability of the obtained gels. Strong correlations were found between the water activity and rheological properties of the gels; an improvement of their rheological properties resulted in a decrease in water activity. The supplementation of pumpkin-seed proteins with egg-white proteins resulted in more homogenous gels with a stronger microstructure and better water binding.Документ Technological functions of hydrolyzed whey concentrate in ice cream(2022) Shevchenko, Oleksandr; Mykhalevych, Artur; Polishchuk, Galyna; Buniowska-Olejnik, Magdalena; Bass, Oksana; Bandura (Kuzmyk), UlianaBased on the calculation of the degree of sweetness of whey concentrates, taking into account the mass fraction of total solids, the degree of lactose hydrolysis and the known values of the relative sweetness of sugar, lactose, glucose and galactose, a concentrate with a solids content 40% was chosen for use in the ice cream formulation. According to the results of the study of the quality indicators of ice cream mixtures, it was found that the hydrolyzed concentrate of demineralized whey with a mass fraction of solids 40% could replace up to 42% of sugar in the omposition of ice cream, while maintaining the degree of sweetness determined for this type of ice cream in the range from 0.8 to 0.9. According to the viscosity-speed characteristics, the mixture of low-fat ice cream with a сoncentrate of hydrolyzed demineralized whey is classified as a system with a pronounced coagulation structure with the detection of thixotropic properties. Ice cream based on hydrolyzed whey concentrate contains 3.3% of whey proteins, which corresponds to the standard chemical composition of ice cream. The high content of lactose hydrolysis products in ice cream increases overrun, but reduces the resistance to melting of ice cream, which must be taken into account during the technological process and when choosing a consumer container. На основі розрахунку ступеня солодкості сироваткових концентратів з урахуванням масової частки загальних сухих речовин, ступеня гідролізу лактози та відомих значень відносної солодкості цукру, лактози, глюкози та галактози, для використання в рецептурі морозива було обрано концентрат з вмістом сухих речовин 40%. За результатами дослідження показників якості сумішей для морозива встановлено, що гідролізований концентрат демінералізованої сироватки молочної з масовою часткою сухої речовини 40 % здатний замінити до 42 % цукру у складі морозива, а збереження ступеня солодкості, визначеного для цього виду морозива в діапазон від 0,8 до 0,9. За в’язкісно-швидкісними характеристиками суміш нежирного морозива з концентратом гідролізованої демінералізованої сироватки класифікують як систему з вираженою коагуляційною структурою з виявленням тиксотропних властивостей. Морозиво на основі концентрату гідролізованої сироватки містить 3,3% сироваткових білків, що відповідає стандартному хімічному складу морозива. Високий вміст у морозиві продуктів гідролізу лактози збільшує перебіг, але знижує стійкість до танення морозива, що необхідно враховувати під час технологічного процесу та при виборі споживчої тари.Документ Study of water freezing in low-fat milky ice cream with oat β-glucan and its influence on quality indicators(2023) Buniowska-Olejnik, Magdalena; Mykhalevych, Artur; Polishchuk, Galyna; Sapiga, Victoria; Znamirowska-Piotrowska, Agata; Kot, Anna; Kaminska-Dwórznicka, AnnaThe work is devoted to the study of the functional and technological properties of oat β-glucan in low-fat milky ice cream (2% fat) in comparison with the stabilization system Cremodan® SI 320. β-glucan (0.5%) has a greater effect on the cryoscopic temperature of ice cream mixes than Cremodan® SI 320 in the same amount (decrease by 0.166 ◦C vs. 0.078 ◦C), which inhibits the freezing process of free water in ice cream during technological processing in the temperature range from −5 to −10 ◦C. Microscopy of ice cream samples after freezing and hardening shows the ability of β-glucan to form a greater number of energy bonds due to specific interaction with milk proteins. Analysis of the microstructure of ice cream samples during 28 d of storage confirms the ability of oat β-glucan to suppress the growth of ice crystals more effectively than Cremodan® SI 320. Oat β-glucan gives ice cream a rich creamy taste, increases overrun and resistance to melting, which brings this type of frozen dessert closer to a full-fat analogue (10% fat).Документ Application of milk protein concentrates in preparation of reduced fat sour cream(2022) Mykhalevych, Artur; Kostenko, Olena; Polishchuk, Galyna; Bandura (Kuzmyk), UlianaThe rational doses of milk-protein ingredients in reduced fat sour cream that prevent excessive acidity of milk cream during fermentation, structure and stabilize this product during 5 days of storage, are the following: skimmed milk powder, 1%, sodium caseinate, 0.5%, caseinate calcium, 0.75%, whey protein concentrate, 1%, hydrolyzed whey concentrate, 30%. According to the level of inhibition of the lactic acid fermentation, milk-protein concentrates in the specified quantities can be arranged in the following sequence: skimmed milk powder → whey protein concentrate → caseinate calcium → hydrolyzed whey concentrate → sodium caseinate. By microstructural analysis of reduced fat sour cream, it was determined that 1% whey protein concentrate ensures proper moisture binding in the sour milk clot and contributes to the formation of a delicate structure with finely dispersed cells, while the use of 30% hydrolyzed whey concentrate forms a more viscous consistency of the product due to the presence of monosaccharides in it, which have a higher adsorption capacity for free moisture. The greatest structuring ability of caseinates and the most significant influence of whey proteins on the thixotropic properties of the reduced fat sour cream have been proved. A comprehensive indicator of the quality of reduced fat sour cream with milk-protein concentrates was calculated. Samples with 1% whey protein concentrate and 30% hydrolyzed whey concentrate had the most attractive sensory indicators, and got the highest score. The chemical composition of reduced fat sour cream samples with whey proteins was studied. It was found that 1% of whey protein concentrate increases the biological value by 1.3%, while 30% of hydrolyzed whey concentrate decreases it by 3.5%. According to the research results, whey protein concentrate was classified as a biological enhancer with moderate technological properties, and hydrolyzed whey concentrate as an effective technological additive that imitates the quality indicators of an analogue with medium fat content of 18-20%. Раціональними дозами молочно-білкових інгредієнтів у складі низькожирної сметани, що запобігають перекисанню вершків, структурують та стабілізують цей продукт впродовж 5-ти діб зберігання є наступні: сухе знежирене молоко – 1%, казеїнат натрію– 0,5%, казеїнат кальцію – 0,75%, концентрат сироваткових білків – 1%, концентрат гідролізованої сироватки – 30%. За ступенем гальмування процесу молочнокислого бродіння молочно-білкові концентрати у вказаних кількостях можна розташувати у такій послідовності: сухе знежирене молоко → концентрат сироваткових білків → казеїнат кальцію → концентрат гідролізованої сироватки → казеїнат натрію. За результатами мікроструктурного аналізу зразків низькожирної сметани визначено, що 1% концентрат сироваткових білків забезпечує належне утримання вологи у кисломолочному згустку та сприяє формуванню ніжної структури з дрібнодисперсними комірками, а використання 30% концентрат гідролізованої сироватки формує більш в’язку консистенцію продукту за наявності в ньому моноцукрів, які мають вищу адсорбувальну здатність вільної вологи. Доведено найбільшу структуруючу здатність казеїнатів та найсуттєвіший вплив сироваткових білків на тиксотропні властивості сметанного продукту. Проведено органолептичну оцінку та розраховано комплексний показник якості зразків низькожирної сметани та визначено зразки з 1% концентрат сироваткових білків та 30% концентратом гідролізованої сироватки як такі, що мають найбільш привабливі органолептичні показники. Досліджено хімічний склад зразків низькожирної сметани з сироватковими білками. Встановлено, що 1% концентрат сироваткових білків підвищує біологічну цінність на 1,3%, в той час як 30% концентрат гідролізованої сироватки знижує на 3,5%. За результатами досліджень, концентрат сироваткових білків було віднесено до біологічного збагачувача з помірними технологічними властивостями, а концентрат гідролізованої сироватки до ефективної технологічної добавки, що імітує показники якості аналогу із середньою жирністю 20%.